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Protease gene methylation as potential marker for early diagnosis of cerebral apoplexy

A methylation and stroke technology, applied in the medical field, can solve problems such as unreported

Pending Publication Date: 2021-12-21
南京腾辰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the relationship between HTRA1 gene methylation in peripheral blood and stroke in the population, and there is no report on the relationship between HTRA1 gene methylation and ischemic stroke or hemorrhagic stroke.
In addition, the relationship between HTRA1 gene methylation and stroke has not been reported in animal models including mice and cell lines

Method used

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  • Protease gene methylation as potential marker for early diagnosis of cerebral apoplexy
  • Protease gene methylation as potential marker for early diagnosis of cerebral apoplexy
  • Protease gene methylation as potential marker for early diagnosis of cerebral apoplexy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0159] Example 1. Primer Design for Detection of HTRA1 Gene Methylation Sites

[0160] After a large number of sequence and functional analysis, this detection covers the methylation level of five fragments (HTRA1_A, HTRA1_B, HTRA1_C, HTRA1_D and HTRA1_E fragments) covering the promoter region, the first exon and the first intron region of the HTRA1 gene Correlation analysis with stroke.

[0161] The HTRA1_A fragment (SEQ ID No.1) is located at chr10:124219200-124221200 of the hg19 reference genome, the sense strand.

[0162] The HTRA1_B fragment (SEQ ID No.2) is located at chr10:124219200-124221200 of the hg19 reference genome, the sense strand.

[0163] The HTRA1_C fragment (SEQ ID No.3) is located at hg19 reference genome chr10:124219200-124221200, antisense strand.

[0164] The HTRA1_D fragment (SEQ ID No.4) is located at chr10:124221500-124223000 of the hg19 reference genome, the sense strand.

[0165] The HTRA1_E fragment (SEQ ID No.5) is located at chr10:124221500-12...

Embodiment 2

[0184]Example 2, HTRA1 gene methylation detection and result analysis

[0185] 1. Research samples

[0186] The epidemiological cluster sampling method was used to conduct a survey of community populations over 18 years old in 16 towns of Jurong City, Jiangsu Province from October to December 2015, with a total of 11,151 people in the baseline survey. This study was reviewed by the Ethics Committee of Nanjing Medical University, and all subjects signed the informed consent.

[0187] The content of the baseline survey includes collecting the general demographic information of the survey object, such as gender, age, place of origin, ethnicity, etc.; asking about disease history, medication history, family history, etc., mainly including history of cardiovascular disease, diabetes, kidney disease, and dyslipidemia; Collect smoking status, drinking status, etc. Smoking status was divided into smoking (defined as current smoking > 20 cigarettes / week, and continuous smoking > 3 mo...

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Abstract

The invention discloses protease gene methylation as a potential marker for early diagnosis of cerebral apoplexy. The invention provides a methylated HTRA1 gene and application of the expression degree of HTRA1 in blood as a marker in preparation of a product. The product has any one of the following functions: (1) auxiliary diagnosis of cerebral apoplexy; and (2) early warning of cerebral apoplexy before clinical symptoms. According to the invention, it is found that the methylation of the HTRA1 in the blood and the expression degree of the HTRA1 in the blood can be used as potential markers for early warning and early diagnosis of the cerebral apoplexy. The invention has important scientific significance and clinical application value for improving the diagnosis and treatment effect of cerebral apoplexy.

Description

technical field [0001] The invention relates to the medical field, in particular to a protease gene methylation as a potential marker for early diagnosis of cerebral apoplexy. Background technique [0002] Stroke is a clinical disease with a series of symptoms caused by local blood circulation disorder in the brain due to sudden rupture of blood supply arteries to the brain or blockage or stenosis of the lumen[Lindgren A.Stroke genetics: a review and update[J].Journal of Stroke, 2014, 16(3): 114]. In 2016, the lifetime risk of stroke among people aged 25 and over in the world was about 25%, and the risk of stroke in the Chinese population was as high as 39.3%, with 41.1% for men and 36.7% for women [Collaborators GBDLRoS, Feigin VL, Nguyen G, Cercy K, Johnson CO, Alam T, et al. Global, regional, and country-specific lifetime risks of stroke, 1990 and 2016. N Engl J Med. 2018; 379:2429-2437]. The "China Cardiovascular Disease Report 2018" pointed out that the number of pati...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883G16B20/20G16B30/00G16B40/00G16H50/20G16H50/70
CPCC12Q1/6883G16B20/20G16B30/00G16B40/00G16H50/20G16H50/70C12Q2600/154
Inventor 杨蓉西沈冲刘春兰尹启明
Owner 南京腾辰生物科技有限公司
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