Detection preparation for detecting atrazine, kit and detection method thereof

A technology of atrazine and a kit, which is applied in the field of detection preparations for detection of atrazine, can solve problems such as influence, and achieve the effects of wide linear range, low detection limit and high sensitivity

Pending Publication Date: 2021-12-21
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional colloidal gold immunochromatographic analysis methods using naked eyes are mostly semi-quantita

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection preparation for detecting atrazine, kit and detection method thereof
  • Detection preparation for detecting atrazine, kit and detection method thereof
  • Detection preparation for detecting atrazine, kit and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0067] Example 1:

[0068] Preparation of a colloidal gold nanoparticle

[0069] The stirring paddle and the glassware were placed in the acid cylinder for overnight, clean and clean; assemble the stirring paddle to the stirrer, turn on the electropump preheating for 5 min; after the water is boiled, 1 ml of 1% sodium citrate is added, and stirred Mixed it; adjust the rotational speed to 500 rpm, and add 1 ml of 1% chlorophytic acid. After the solution becomes red, the heat source is removed, continued to stir; the temperature drops to stop stirring at 50 ° C, and the glue gold capacity bottle is allowed to 100 ml, and the ultraviolet absorption peak is determined.

[0070] Steps Ertraz monoclonal antibody - Preparation and optimization of colloidal gold label

[0071] Take 1 ml OD520 = 1 colloidal gold, add 20 μl of sodium carbonate to about 8.5 to adjust the pH to 8.5, add 7 μl of 1 mg / ml of Atlai, mixing 30 min, add 10% BSA 120 μL, close 15 min, 10000 rpm Centrifuge 15 min Ab...

Example Embodiment

[0073] Example 2 Preparation of a paper strip

[0074] Formulate T-line antigen rowfirvani: contains 1.5 mg / ml of Atlasi complete antigen (Atlai-Ovatorianoabi), 100mm PB buffer of PEG20000 with a mass percentage concentration of 0.5%, pH The value is 6.2;

[0075] The C-line antibody scribe fluid was prepared: a 0.3 mg / mL of sheep anti-mouse antibody was diluted with a PBS solution.

[0076] The T-line antigen rowder, the C wire antibody scribe fluid is divided into the NC film, and the rowline flow rate is 1 μl / cm, and the distance between the T line and the C wire is 5 mm. The drawn NC film was placed in an oven at 37 ° C for 4 h, taken out.

[0077] The Atlau monoclonal antibody labeled colloidal gold prepared in Example 1 was stored on the reaction hole, paste the sample pad, binding pad, NC film, and absorbent pad.

[0078] Specific paste: First, the NC film is first pasted, and then bond the bond pad in the lower end of the NC film, and then overlap the NC membrane, an...

Example Embodiment

[0083] Experimental Example 1 Preparation and Optimization of Atlai Sandel Antibody - Colloid Gold Label

[0084] Take 1 ml of colloidal gold, add 20 μl of sodium carbonate to about 8.5 to 8.5, add 3, 5, 7, 10 μl of Atlasi monocompanion, mixing for 30 min, add 10% BSA 120 μL, close 15 min 10000 rpm was centrifuged for 15 minutes, discarded, 1 ml of antibody stabilization, reconstituted, continued 8000 rpm centrifugation for 15 min, repeated 2 times, and finally 100 μL of antibody stabilizers resuspended to obtain an Atlau monoclonal antibody labeled colloidal gold.

[0085] The prepared Atlai monoclonal antibody labeled colloidal gold was mixed with 50 ppb ATZ, and then dropped into the sample pad of the test strip while setting the blank group (containing only Atlai monoclonal antibody labeled colloids). Gold) as a control. Make Atlai monoclonal antibody labeled colloids in the T-axis complete antigen, using a hand-held card reader to measure the color value on the T line, the ex...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of biological detection, in particular to a detection preparation for detecting atrazine, a kit thereof and a detection method. The detection preparation comprises a colloidal gold test strip and a reaction hole, the colloidal gold test strip comprises a sample pad, an NC membrane and an absorption pad, and a T line and a C line are arranged on the NC membrane; colloidal gold labeled by an atrazine monoclonal antibody is freeze-dried and stored in the reaction hole; the T line is coated with an atrazine complete antigen and is used for competitively combining the colloidal gold labeled by the atrazine monoclonal antibody; and the C line is coated with a goat anti-mouse antibody and is used for capturing uncombined colloidal gold labeled by the atrazine monoclonal antibody. The detection preparation provided by the invention can detect atrazine within 15 minutes, and has the technical advantages of wide linear range, low detection limit and high sensitivity.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection preparation for detecting atrazine, a kit and a detection method thereof. Background technique [0002] Atrazine (ATZ) is also known as atrazine, and its molecular formula is C 8 h 14 ClN 5 . It is a typical triazine herbicide. Atrazine is a selective systemic conduction pre-emergence and post-emergence herbicide. It is mainly used in dry fields such as corn, sorghum, sugarcane, fruit trees, and woodlands to control annual grasses. Weeds and broad-leaved weeds also have a certain inhibitory effect on certain perennial weeds. Although it is a low toxicity herbicide, it is moderately persistent in soil with a half-life of up to 57 weeks. The pollution caused by atrazine due to its stable chemical properties and long residual time has attracted more and more attention. Atrazine was positively detected in surface water, groundwater, soil, and crops. ATZ was found...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/577G01N33/558G01N33/58G01N33/543G01N33/53
CPCG01N33/577G01N33/558G01N33/587G01N33/54346G01N33/5308G01N2430/20
Inventor 孙铁强姚站馨宁保安高蔚娜张予弦郭长江杨涵王新阳
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap