A kind of cryopreservation liquid and its preparation method and application

Abstract

The invention provides a novel low-temperature preservation solution and its preparation method and application. The composition of the low-temperature preservation solution according to the final concentration includes: FBS, 30-70%; Cultrex UltiMatrix RGF BME, 5-15%; trehalose, 1-15%. 5g / L; hydroxyethyl starch, 10‑30g / L; LB‑100, 0.1‑5μM; RGFP109, 10‑100nM; Within the scope of 7.6, the above percentage concentration is volume fraction. The cryopreservation solution is used in cryopreservation of biological samples. Compared with known cryoprotectants, it can better protect cells from damage caused by ice crystals and osmotic pressure, and reduce damage to mitochondria and endoplasmic reticulum stress caused by long-term cryopreservation. Cell apoptosis, while maintaining the stability of intracellular biomacromolecules to the greatest extent.

Description

A kind of cryopreservation liquid and its preparation method and application technical field The present invention relates to biological sample preservation technical field, be specifically related to a kind of novel cryopreservation liquid and preparation method thereof law and application. Background technique In the era of precision medicine, biological samples have been widely used in the treatment of tumors, immune system diseases, diabetes Diabetes and new drug development and other biomedical fields. Samples such as umbilical cord blood, sperm eggs and stem cells need to be stored for a long time The activity and function after rewarming are not affected before it can be used in clinical treatment. Emerging development of stem cell therapy, gene therapy Biological treatments such as therapy and immunotherapy also require high-quality biological samples as a basis. as preservation of biological samples Biological information and clinical data are used in s...

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Problems solved by technology

However, cryoprotectants can produce cytotoxicity when the temperature is near the freezing point, and lipophilic cryoprotectants are usually also cytotoxic, which seriously affects the activity of biological samples after cryopreservation and recovery, and causes waste of samples and reagents

[0005] In addition, there are still many problems to be solved in the current cryopreservation technology: 1) Due to the instability of RNA and protein, long-term storage may cause degradation to varying degrees; 2) Adding cryoprotectant preservation can keep cells However, due to ice crystal damage, osmotic pressure damage and the addition of foreign substances such as protective agents, it will inevitably bring a certain degree of damage to the cells; 3) the impact of low temperature on the morphology and function of cells and tissues; 4) the impact of low temperature on DNA , RNA and protein and other biological macromolecules

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