Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for simultaneously extracting polysaccharide and alkaloid in dendrobium officinale

A technology of Dendrobium candidum and alkaloids, applied in the direction of alkaloids, alkaloids, chemical instruments and methods, etc., can solve the problems of low extraction rate, organic solvent extraction pollution, enzymatic hydrolysis residue, etc., and achieve high extraction rate and high extraction rate rate, the effect of reducing the tendency of aggregation

Active Publication Date: 2021-12-31
大冶康之堂农业发展有限公司
View PDF13 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also a small number of documents that pay attention to the simultaneous extraction of alkaloids and polysaccharides, such as patent documents CN111253495A, CN104448013A, CN103113483A, etc., but there are deficiencies such as enzymatic hydrolysis residue, organic solvent extraction pollution, and the problem of low extraction rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously extracting polysaccharide and alkaloid in dendrobium officinale

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for simultaneously extracting polysaccharides and alkaloids in Dendrobium officinale comprises the following steps:

[0032] (1) pretreatment: the Dendrobium candidum to be treated is cleaned, dried, then crushed to 60 mesh (quality 50g), for subsequent use;

[0033] (2) Surfactant infiltration: place the Dendrobium officinale pretreated in step (1) in 25 mL of OP-10 solution with a mass concentration of 0.5%, and infiltrate at room temperature for 0.5 h;

[0034] (3) Steam blasting: the Dendrobium candidum after soaking in step (2) is quickly placed in the steam blasting machine QBS-80, and then 1.2Mpa of water vapor is introduced to carry out steam blasting. The pressure maintenance time is 150s. Quickly release the pressure to get blasting materials;

[0035] (4) Microwave treatment: add water to the blasting material obtained in step (3) according to the mass ratio of 1:12, adjust the pH value of the system to 3.0 by adding citric acid, stir and mix well, ...

Embodiment 2

[0039] A method for simultaneously extracting polysaccharides and alkaloids in Dendrobium officinale comprises the following steps:

[0040] (1) pretreatment: the Dendrobium candidum to be treated is cleaned, dried, then crushed to 60 mesh (quality 50g), for subsequent use;

[0041] (2) Surfactant infiltration: place the Dendrobium officinale pretreated in step (1) in 25 mL of OP-10 solution with a mass concentration of 0.7%, and infiltrate at room temperature for 1 h;

[0042] (3) Steam blasting: the Dendrobium candidum after soaking in step (2) is quickly placed in the steam blasting machine QBS-80, and then 1.2Mpa of water vapor is introduced to carry out steam blasting. The pressure maintenance time is 150s. Quickly release the pressure to get blasting materials;

[0043] (4) Microwave treatment: Add water to the blasting material obtained in step (3) according to the mass ratio of 1:14, adjust the pH value of the system to 3.4 by adding citric acid, stir and mix well, then...

Embodiment 3

[0047] A method for simultaneously extracting polysaccharides and alkaloids in Dendrobium officinale comprises the following steps:

[0048] (1) pretreatment: the Dendrobium candidum to be treated is cleaned, dried, then crushed to 60 mesh (quality 50g), for subsequent use;

[0049] (2) Surfactant infiltration: place the Dendrobium officinale pretreated in step (1) in 25 mL of OP-10 solution with a mass concentration of 0.8%, and infiltrate at room temperature for 1 h;

[0050] (3) Steam blasting: the Dendrobium candidum after soaking in step (2) is quickly placed in the steam blasting machine QBS-80, and then 1.2Mpa of water vapor is introduced to carry out steam blasting. The pressure maintenance time is 150s. Quickly release the pressure to get blasting materials;

[0051] (4) Microwave treatment: Add water to the blasting material obtained in step (3) according to the mass ratio of 1:16, adjust the pH value of the system to 3.8 by adding citric acid, stir and mix well, th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for simultaneously extracting polysaccharide and alkaloid in dendrobium officinale. The method comprises six steps of pretreatment, surfactant infiltration, steam explosion, microwave treatment, flocculation, membrane filtration separation and the like. According to the invention, the combined extraction method mainly adopting a surfactant and steam explosion is creatively adopted, and microwave, flocculation, membrane separation and other processes are combined, so that polysaccharide and alkaloid in dendrobium officinale can be fully extracted at the same time, the extraction rate is high, the defects of solvent residue, material structure damage and the like caused by extraction, enzymolysis and other treatments of a large amount of organic solvents are overcome, and the effect is obvious. The extraction rate of alkaloid exceeds 0.03%, the extraction rate of polysaccharide exceeds 27%, the purity of alkaloid and polysaccharide is high, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of resource utilization of dendrobium candidum, and more specifically relates to a method for simultaneously extracting polysaccharides and alkaloids in dendrobium candidum. Background technique [0002] Dendrobium officinale is a herbaceous plant of the genus Dendrobium widely distributed in Hubei, Guizhou, Guangxi, Yunnan and other places in my country. Modern pharmacological studies have shown that Dendrobium candidum has anticoagulant, anti-tumor, lowering three highs, anti-aging, and improving immunity effects. Therefore, traditionally, Dendrobium candidum is directly processed as a traditional Chinese medicine ingredient. However, this method is primitive and The development and utilization of the medicinal value of Dendrobium candidum is not enough. Therefore, with the development of biomedical technology, the extraction and utilization of the main components in Dendrobium officinale have attracted a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07G5/00
CPCC08B37/0003C07G5/00
Inventor 冯海明冯伟华黄春霞崔丙存洪海林刘先辉
Owner 大冶康之堂农业发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com