Intestinal biomarkers for gut health in domesticated birds

A technology for intestinal health and intestinal tract, applied in biological testing, biochemical equipment and methods, microbial determination/inspection, etc.

Pending Publication Date: 2021-12-31
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are currently no quantifiable and easily measurable biomarkers for diagnosing or predicting gut health in poultry, but such biomarkers are useful for monitoring and/or predicting causes of or associated with flock performa

Method used

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  • Intestinal biomarkers for gut health in domesticated birds
  • Intestinal biomarkers for gut health in domesticated birds
  • Intestinal biomarkers for gut health in domesticated birds

Examples

Experimental program
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example 1

[0055] Example 1: Determination

[0056]In the following examples, the various assays described below were used for ease of reading. Any deviations from the protocol provided below are indicated in the relevant sections. In these experiments, a spectrophotometer is used to measure the absorbance of the product formed after the reaction is complete.

[0057] Histology: Duodenal rings were fixed in 4% formaldehyde for 24 h, dehydrated in xylene and embedded in paraffin. Sections of 4 μm were cut using a microtome (Microme HM360, Thermo Scientific) and processed as described by De Maesschalck et al. (2015). The villus length and occult length in the duodenum were determined by randomly measuring twelve villi / gut segments using a standard light microscope (Leica DM LB2Digita) and the computer-based image analysis program LAS V4.1 (Leica Application Suite V4, Germany). nest depth. The ratio of villi to crypts was then calculated. Antigen retrieval was performed on 4 μm duode...

example 2

[0069] Example 2: Inducing microecological imbalance in chickens with provocative model tests

[0070] A total of 360-day-old broiler chickens (Ross 308) were obtained from a local hatchery and housed in floor pens on wood shavings. Feed and drinking water were provided ad libitum throughout the study. Broilers were randomly assigned to two treatment groups, a control group and a challenge group (9 pens / treatment, 20 broilers / pen). All animals were fed commercial feed until day 12, and feed was switched to a wheat-based (57.5%) diet supplemented with 5% rye. From day 12 to day 18, all animals from the challenge group received 10 mg florfenicol / kg body weight and 10 mg enrofloxacin / kg body weight daily via drinking water to induce substantial changes in the gut microflora. After antibiotic treatment, give 1 ml daily by oral gavage from day 19 to 21 from 10 9 cfu Escherichia coli (G.78.71), 10 10 cfu Enterococcus species (G.78.62), 10 9 cfu Lactobacillus salivarius (LMG22...

example 3

[0074] Example 3: Identification of metabolic biomarkers associated with gut health

[0075] Metabolomic analysis was performed on colon and cecum samples from control and challenged animals of Example 2. Such as Figure 6A and Figure 6B As shown in the stimulated chicken colon ( Figure 6A ) and cecum ( Figure 6B ) were observed at significantly higher levels compared to their corresponding levels in control chickens. Apart from Figure 6A and Figure 6B In addition to the metabolites shown in , the following additional compounds were found in the gut of challenged chickens at significantly higher levels than those found in unchallenged controls: linoleoylcarnitine, linalool, 3 -[(9Z)-9-octadecenoyloxy]-4-(trimethylammonium)butyrate, (-)-trans-methyldihydrojasmonate, icomerin, 1,3 -Dicaprylylglycerol and ethyl 2-nonanoate. Thus, the presence of one or more of these compounds at levels significantly higher than in healthy control animals is associated with poor gut...

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Abstract

Provided herein, inter alia, are methods for measuring and assessing intestinal health in poultry. The disclosed metabolic biomarkers and associated methods for identifying and quantifying the same are reliable, rapid and, in some embodiments, non-invasive, and can be used to provide information with respect to the gut health of poultry, such as chickens.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Application No. 62 / 827606, filed April 1, 2019, which is hereby incorporated by reference in its entirety. technical field [0003] In particular, methods for measuring and assessing the gut health of domestic poultry are provided herein. Background technique [0004] In poultry species, the gastrointestinal tract is not only involved in digestion and absorption, but also interacts with the immune system to promote good health. The lumen of the intestine is coated with a thin, thick layer of mucus, and millions of bacteria and other microorganisms are embedded in the mucus layer. When gut bacteria are in balance (that is, good bacteria outnumber bad ones), the digestive tract can be said to be in a healthy state. A healthy microbiota provides multiple benefits to the host, including resistance to colonization by a broad spectrum of pathogens, biosynthesis and absorpt...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12Q1/689C12Q1/6888G01N33/50
CPCC12Q1/04C12Q1/6883C12Q1/689G01N33/5308G01N33/5091
Inventor A·贝克勒-伊特巴雷克M·伯纳多V·伊克豪特K·吉布斯F·凡伊梅塞尔
Owner DUPONT NUTRITION BIOSCIENCES APS
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