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Self-dissolving solution for novel lysobacter enzymogenes strain LE16 and preparation method of self-dissolving solution

A LE16, self-solution technology, applied in microorganism-based methods, biochemical equipment and methods, microbial dissolution, etc., to achieve good results, less resource consumption, and low industrial production requirements.

Pending Publication Date: 2022-01-07
ZUNYI TOBACCO OF GUIZHOU TOBACCO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are strict requirements for the autolysis of LE16. The formula of the medium, the volume of the culture tank, the amount of liquid, the culture temperature, etc., there is no process and application technology for the preparation of the self-solution at home and abroad.

Method used

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  • Self-dissolving solution for novel lysobacter enzymogenes strain LE16 and preparation method of self-dissolving solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The self-solution used for the new strain LE16 of Enzymolyticus in this embodiment, the self-solution raw material comprises the following components by weight: 10 parts of peptone, 5 parts of beef extract, 5 parts of NaCl, 0.5 part of tryptophan betaine, Tianmen 0.5 parts of paraparagine, 0.05 parts of multivitamins, (NH 4 ) 2 SO 4 1 part, 5 parts of glucose, KH 2 PO 4 1 part, MgSO 4 0.5 servings.

[0020] This self-solution for the new strain LE16 of Lysobacterium enzymolyticus is used for preparing LE16 self-solution, comprises the following steps: a. Seed tank culture medium: Peptone (6 parts), beef extract (4 parts), NaCl part, tryptophan Betaine (0.25 parts), asparagine (0.25 parts), and vitamin B complex (0.0025 parts) were dissolved in distilled water, and the initial pH was 7.0 to 7.2; the seed tank culture solution was prepared;

[0021] b. Fermentation tank culture medium: peptone (4 parts), (NH 4 ) 2 SO 4 , beef extract (1 part), glucose, KH 2 PO ...

Embodiment 2

[0026] The self-solution for the new strain LE16 of zymolytic bacillus in this embodiment, the self-solution raw material comprises the following components by weight: 20 parts of peptone, 15 parts of beef extract, 15 parts of NaCl, 3 parts of tryptophan betaine, asparagus amide 3 parts, multivitamin 0.5 part, (NH 4 ) 2 SO 4 10 parts, glucose 15 parts, KH 2 PO 4 5 parts, MgSO 4 3 copies.

[0027] This self-solution for the new strain LE16 of Lysobacterium zymolyticus is used to prepare LE16 self-solution, comprising the following steps: a. Seed tank culture medium: peptone (15 parts), beef extract (10 parts), NaCl, tryptophan beet Alkali (1.5 parts), asparagine (1.5 parts), and vitamin B complex (0.25 parts) were dissolved in distilled water, and the initial pH was 7.0 to 7.2; the seed tank culture solution was prepared;

[0028] b. Fermentation tank culture medium: peptone (5 parts), (NH 4 ) 2 SO 4 , beef extract (5 parts), glucose, KH 2 PO 4 , NaCl, MgSO 4 , tryp...

Embodiment 3

[0033] The self-solution for the new strain LE16 of Enzymolytica of the present embodiment, the self-solution raw material comprises the following components by weight: 10 parts of peptone, 15 parts of beef extract, 5 parts of NaCl, 3 parts of tryptophan betaine, Tianmen 0.5 parts of paraparagine, 0.5 parts of multivitamins, (NH 4 ) 2 SO 4 1 part, glucose 15 parts, KH 2 PO 4 1 part, MgSO 4 3 copies.

[0034] This self-solution for the new strain LE16 of Lysobacterium zymolyticum is used to prepare LE16 self-solution, comprising the following steps: a. Seed tank culture medium: peptone (7 parts), beef extract (11 parts), NaCl, tryptophan beet Alkali (1.5 parts), asparagine (0.25 parts), and vitamin B complex (0.25 parts) were dissolved in distilled water, and the initial pH was 7.0 to 7.2; the seed tank culture solution was prepared;

[0035] b. Fermentation tank culture medium: peptone (3 parts), (NH 4 ) 2 SO 4 , beef extract (4 parts), glucose, KH 2 PO 4 , NaCl, Mg...

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Abstract

The invention discloses a self-dissolving solution for a novel lysobacter enzymogenes strain LE16. The self-dissolving solution is prepared from the following raw material components in parts by weight: 10 to 20 parts of peptone, 5 to 15 parts of beef extract, 5 to 15 parts of NaCl, 0.5 to 3 parts of tryptophan betaine, 0.5 to 3 parts of asparagine, 0.05 to 0.5 part of multivitamins, 1 to 10 parts of (NH4)2SO4, 5 to 15 parts of glucose, 1 to 5 parts of KH2PO4 and 0.5 to 3 parts of MgSO4. The prepared self-dissolving solution of the LE16 does not contain thalli, does not need filtration sterilization, has a good and stable effect of preventing and treating powdery mildew and gray mold of plants, is long in storage time, is slightly influenced by conditions such as environments, and is simple in application method, high in practicability and easy to popularize, a technical system of a preparation process is simple and universal in equipment, low in resource consumption and low in industrial production requirements, and a general microbial preparation preparing factory can meet the production requirements.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to a self-solution for a new strain of zymolysobacillus LE16 and a preparation method thereof. Background technique [0002] As a typical representative of the genus Lysobacter, Lysobacter enzymogenes has the ability to secrete a variety of hydrolytic enzymes, which is also the reason for its name. At present, the lysobacterium enzymogenes that have been produced and applied include lysobacterium enzymogenes C3 (L.enzymogenes C3), lysobacterium enzymogenes 3.1T8 (L.enzymogenes 3.1T8), lysobacterium enzymogenes N4-7 (L. 7) and L. enzymogenes OH11, etc., all play an important role in the control of crop diseases. A new enzyme-producing lysobacterium strain L.enzymogenes LE16 (hereinafter referred to as LE16) has the functions of promoting growth and preventing diseases. At present, the strain is preserved in the General Microbiology Center of the China Committee for Microbial Culture Colle...

Claims

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Application Information

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IPC IPC(8): C12N1/06C12R1/01
CPCC12N1/06
Inventor 黄纯杨漆夏燕李治模董祥立彭玉龙温明霞王小彦杨红军黄建国
Owner ZUNYI TOBACCO OF GUIZHOU TOBACCO CORP
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