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Composite solid biological preparation based on self-assembled crystalline material, and preparation and application thereof

A biological preparation, self-assembly technology, applied in the application of composite solid biological preparations in different fields, self-assembled crystalline material composite solid biological preparations and its preparation field, can solve problems such as the immobilization technology of complex liquid biological product systems

Pending Publication Date: 2022-01-11
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no immobilization technology for complex liquid biological product systems

Method used

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  • Composite solid biological preparation based on self-assembled crystalline material, and preparation and application thereof
  • Composite solid biological preparation based on self-assembled crystalline material, and preparation and application thereof
  • Composite solid biological preparation based on self-assembled crystalline material, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The preparation of the in-situ encapsulation method to load biological agents @MOFs, the specific implementation steps are as follows:

[0064] Preparation of biological preparation @ UTSA-280 solid preparation: Weigh 32 mg of sodium squarylate trihydrate and dissolve in 1 ml of deionized water. Weigh 236 mg of calcium nitrate tetrahydrate and dissolve in 1 ml of deionized water. Dilute the biological agent solution to an appropriate multiple, take 2 ml into a test tube, and add 1 ml of prepared sodium squarylate solution and calcium nitrate solution respectively. After standing at room temperature for 1 hour, a white precipitate was obtained. The white precipitate was collected by centrifugation at 4000r, washed 3 times with water, freeze-dried and placed in a 4°C refrigerator for use. Biological agents here include fetal bovine serum and horse serum.

[0065] Preparation of biological agent@MDP-Zn solid preparation: Weigh 60 mg of sodium dicyanamide and 31 mg of py...

Embodiment 2

[0075] Preparation of biological agents @COFs by adsorption method, the specific implementation steps are as follows:

[0076] TPA-DMTP: Weigh the ligands tris(4-aminophenyl)amine (TPA 11.6mg) and 2,5-dimethoxybenzene-1,4-dicarbaldehyde (DMTP 11.7mg) and add them to thick-walled heat-resistant Add 0.2mL o-dichlorobenzene, 0.8mL n-butanol and 0.2mL, 3M acetic acid to the glass tube, then quickly freeze in liquid nitrogen, then vacuumize, and then seal the tube with a flame of an oxygen hydrogen machine. Put the sealed glass tube in an oven at 120°C for 3 days.

[0077] TPB-DMTP: Weigh the ligands 1,3,5-tris(4-nitrophenyl)benzene (TPB 14,1 mg) and 2,5-dimethoxybenzene-1,4-dicarbaldehyde (DMTP 11.7mg) into a thick-walled heat-resistant glass tube, then add 0.6mL o-dichlorobenzene, 0.6mL n-butanol and 0.25mL, 3M acetic acid, then quickly freeze in liquid nitrogen, then vacuumize, and then use a hydrogen oxygen machine flame Seal tube. Put the sealed glass tube in an oven at 120...

Embodiment 3

[0082] To test the cytotoxicity of three kinds of MOFs, the specific implementation steps are as follows:

[0083] According to the concentration of the material after dissolution and release, a 1 mg / ml material solution was taken and 10% of the volume of the cell culture medium was added to the material solution. Cells cultured in normal serum were set as the control group, and cells cultured in basal medium were set as the blank group. Observe the growth state of the cells for five days, and make an attached Figure 6 Cell growth curves are shown.

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PUM

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Abstract

Aiming at the defects of the traditional liquid biological preparation, a self-assembled crystalline material is creatively utilized to prepare a composite solid biological preparation. The self-assembled crystalline material can protect biomolecules from being interfered by an external environment, improve the storage stability and maintain the activity of the biomolecules to the maximum extent. Meanwhile, the prepared biological preparation can release the biomolecules under mild conditions, and the released biomolecules can still keep the original activity. The method provided by the invention has the advantages of simple preparation method, low storage cost, high stability and high release rate, and does not influence the activity of the biomolecules.

Description

technical field [0001] The invention relates to the technical field of selective separation and immobilization of biological preparations, in particular to a highly biocompatible self-assembled crystalline material composite solid biological preparation and a preparation method thereof, as well as the application of the composite solid biological preparation in different fields. Background technique [0002] There are a variety of biological agents in nature, including biological macromolecules such as proteins, carbohydrates, lipids, and nucleic acids, as well as some biological small molecules. Biological agents have great application value, such as cell culture, fermentation, chemical industry, food and other fields are inseparable from biological agents. However, biological agents are usually produced by living organisms, and artificial synthesis is difficult to obtain, especially liquid biological agents, which are difficult to store, easy to inactivate, sensitive to th...

Claims

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Application Information

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IPC IPC(8): A61K47/24A61K47/54A61K47/18
CPCA61K47/24A61K47/545A61K47/18A61K47/54
Inventor 陈瑶宋杰张赛男张振杰
Owner NANKAI UNIV