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Universal organ chip perfusion device

An organ chip, a general-purpose technology, applied in biochemical cleaning devices, enzymology/microbiology devices, tissue cell/virus culture devices, etc. Effects of sterilization probability, volume reduction, and simple operation

Pending Publication Date: 2022-01-14
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole device is bulky and complicated
The complexity of device and operation limits the development of organ-on-a-chip and its wide acceptance in biological laboratories

Method used

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  • Universal organ chip perfusion device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Effects of PM2.5 Particles on Lung Epithelium

[0035] Utilize common sandwich chip to simulate lung barrier function. Put 1 lung chip, 1 50ml liquid reservoir, 1 50ml waste liquid reservoir, 1 peristaltic pump at the specified position on the assembly plate with a length of 10cm, a width of 10cm, and a thickness of 1cm, and connect them together through a silicone tube ( Figure 4 ) to sterilize the assembly plate as a whole. Store the appropriate amount of medium in the reservoir and fill the pump tubing with medium. Lung epithelial cells were inoculated on the upper layer of the lung chip sandwich, and vascular endothelial cells were inoculated on the lower layer. Then place the assembly plate as a whole in the incubator and let it stand for 2 hours. After the cells adhere to the wall, connect the peristaltic pump to the control system. And input the flow rate parameter of 50ul / h in the control system, start the peristaltic pump to drive the fluid. Put the assem...

Embodiment 2

[0037] Effects of Alcohol on the Intestinal Epithelium

[0038] Utilize common sandwich chip to simulate lung barrier function. Put 2 intestinal chips, 3 30ml liquid storage tanks, 3 30ml waste liquid tanks, and 3 peristaltic pumps on the designated positions of the 20cm long, 20cm wide, 3cm thick manifold and connect them together through silicone tubes ( Figure 4 ) to sterilize the assembly plate as a whole. Store the appropriate amount of medium in the reservoir and fill the pump tubing with medium. Intestinal epithelial cells were inoculated on the upper layer of the intestinal chip sandwich, and vascular endothelial cells were inoculated on the lower layer. Then place the assembly plate as a whole in the incubator and let it stand for 2 hours. After the cells adhere to the wall, connect the peristaltic pump to the control system. And input the flow rate parameter of 600ul / h in the control system, start the peristaltic pump to drive the fluid. Put the assembly plate ...

Embodiment 3

[0040] Effects of coronaviruses on the lung epithelium

[0041] Utilize common sandwich chip to simulate lung barrier function. Put 3 lung chips, 3 20ml liquid storage tanks, 3 20ml waste liquid tanks, and 3 peristaltic pumps on the designated positions of the 20cm long, 20cm wide, 2cm thick manifold and connect them together through silicone tubes ( Figure 4 ) to sterilize the assembly plate as a whole. Store the appropriate amount of medium in the reservoir and fill the pump tubing with medium. Lung epithelial cells were inoculated on the upper layer of the lung chip sandwich, and vascular endothelial cells were inoculated on the lower layer. Then place the assembly plate as a whole in the incubator and let it stand for 2 hours. After the cells adhere to the wall, connect the peristaltic pump to the control system. And input the flow rate parameter of 1000ul / h in the control system, start the peristaltic pump to drive the fluid. Put the assembly plate into the incubato...

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Abstract

The invention discloses a universal organ chip perfusion device. The device consists of a control system and a packaging flat plate, wherein the packaging flat plate is used for fixing peristaltic pumps, organ chips, a liquid storage pool and a waste liquid pool. The perfusion device is suitable for organ chips in various forms, and the peristaltic pumps are used as a driving device for fluid driving. Elements required for perfusion of a traditional organ chip are integrated on one packaging flat plate, so that the usability of the organ chips is improved, and the perfusion process of the organ chips is simplified. Besides, the packaging flat plate provided with the peristaltic pumps, the organ chips, the liquid storage pool and the waste liquid pool can be integrally sterilized, and the cells are integrally put into the incubator after being operated, so that the cell contamination probability is reduced.

Description

technical field [0001] The invention relates to the field of organ chip perfusion culture. Specifically relates to a general organ chip perfusion system. Background technique [0002] A large amount of funds are wasted every year in the world on the screening of failed drugs. Currently, commonly used drug screening models include cell models and animal models. There are species differences between animal models and humans, which cannot predict the efficacy of drugs well. Cell models are often a single type of cell and are cultured in two dimensions, which cannot reproduce the interaction between organs. [0003] Organ chip is an emerging cutting-edge science and technology, and it is also a typical multi-disciplinary cross-convergence technology. It has important strategic significance for human health and the development of the bio-industry. It has attracted the attention of the government, the scientific community and the industry. Davos World Economic Forum listed as ...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/36C12M1/34C12M1/00
CPCC12M21/08C12M29/06C12M41/48
Inventor 秦建华邓鹏伟
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI