Method for creating diploid germplasm resources by using tetraploid Chinese cabbages

A tetraploid and Chinese cabbage technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problem of slow self-homozygous speed of tetraploid Chinese cabbage, homogeneity of diploid Chinese cabbage resources, etc. question

Active Publication Date: 2022-01-18
河北省农林科学院经济作物研究所
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Problems solved by technology

[0005] In view of this, the present invention has established a new method for utilizing the free microspores of tetraploid Chinese cabbage to create a diploid germplasm resource that is completely different from the parent, to solve the problem of slow self-homozygous speed and large diploid of tetraploid Chinese cabbage. Homogenization of Cabbage Resources

Method used

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  • Method for creating diploid germplasm resources by using tetraploid Chinese cabbages
  • Method for creating diploid germplasm resources by using tetraploid Chinese cabbages
  • Method for creating diploid germplasm resources by using tetraploid Chinese cabbages

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Experimental program
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Embodiment 1

[0044] 1. Chromosome doubling in diploid Chinese cabbage

[0045] (1) Pregermination: Take 1g of the seeds of the diploid Chinese cabbage inbred line 'Fanxinbai', soak the seeds in warm soup at 55°C, wrap them with damp soft gauze, and germinate them in a 28°C thermostat for 12-20 hours, at 80% It is advisable for the seed coat to crack and spit out white.

[0046] (2) Sowing: Use 50 hole trays to sow 2 trays, sow 3-4 seeds in each hole (guarantee 1 seed for germination), sow at a depth of 1cm, cover the substrate and cover with plastic film to keep moisture, in an artificial climate room at 25°C and light intensity Seedlings were grown under 1000-1500lx, and the plastic film was removed in time after the seedlings broke through the soil.

[0047] (3) Double treatment: When the cotyledons are unfolded, put the absorbent cotton balls with a diameter of 3-5 mm in the middle of the cotyledon tightly against the growth point, drip the absorbent cotton balls with 0.2% colchicine s...

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Abstract

The invention relates to the technical field of Chinese cabbage breeding, and particularly relates to a method for creating diploid germplasm resources by using tetraploid Chinese cabbages. The tetraploid Chinese cabbages are subjected to isolated microspore culture, the obtained tetraploid isolated microspores are subjected to heat shock treatment and dark culture temperature regulation culture to obtain embryoids, and the embryoids are subjected to tissue culture and acclimatization and transplantation to obtain regenerated plants and germplasm resources. According to the method, the advantage that the number of chromosomes of the tetraploid Chinese cabbages is multiplied is utilized, the frequency of interchromosomal gene interchanges is significantly higher than the frequency of diploid intergenic interchanges, and the formed microspore genotypes are richer, so that a new diploid material with more variant characters can be obtained.

Description

technical field [0001] The invention relates to the technical field of Chinese cabbage breeding, in particular to a method for creating diploid germplasm resources from tetraploid Chinese cabbage. Background technique [0002] Chinese cabbage is a crop native to China, and it is one of the main cruciferous vegetables. Diploid varieties are generally used in production. Its chromosomes are two sets of 20 chromosomes in total, and the number of gamete chromosomes is n=10. At present, the creation methods of Chinese cabbage germplasm resources include: selfing, hybridization, backcrossing, distant hybridization and other forms. In view of the fact that under the traditional Chinese cabbage breeding technology, it is becoming more and more difficult to create new resources, and the germplasm resources mastered by breeders tend to be homogeneous. Creating new germplasm resources through innovative breeding technologies has become a Chinese cabbage breeding strategy. urgent needs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
CPCA01H4/008C12N5/0025C12N5/04
Inventor 刘晓东王明秋孟川吴芳王玉海牟金贵陈占良马蕾
Owner 河北省农林科学院经济作物研究所
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