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Talaromyces pinophilus NJAU-L8 for preventing and controlling continuous crop rotation soil-borne wilt disease and use thereof

A technology of NJAU-L8, banana wilt, which is applied in the direction of chemicals, applications, fungi, etc. for biological control, and can solve problems such as high economic costs and promotion obstacles

Active Publication Date: 2022-01-18
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the various constraints of chemical fungicides, the use of chemical fungicides should be reduced in the future, and although the crop rotation mode can significantly suppress the incidence of Fusarium wilt, due to its relatively high economic cost, there are still difficulties in the promotion of this method. big obstacle

Method used

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  • Talaromyces pinophilus NJAU-L8 for preventing and controlling continuous crop rotation soil-borne wilt disease and use thereof
  • Talaromyces pinophilus NJAU-L8 for preventing and controlling continuous crop rotation soil-borne wilt disease and use thereof
  • Talaromyces pinophilus NJAU-L8 for preventing and controlling continuous crop rotation soil-borne wilt disease and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and screening of inhibiting Fusarium oxysporum fungi

[0029] Test procedure

[0030] 1. Test materials:

[0031] Pathogen tested: Fusarium oxysporum f.sp.cubense tropical race 4 (Foc4), the pathogen of banana wilt Key laboratories are separated and preserved.

[0032] Soil for test: Soil of high diseased banana orchard in Jianfeng Town, Ledong County, Hainan.

[0033] Test medium: PDA medium (g / L): 200.0g potato, 20.0g glucose, 18.0g agar, natural pH; NA medium (g / L): 5.0g NaCl, 3.0g beef extract, 10.0g peptone , pH 7.0-7.2.

[0034] Main reagents and equipment: Premix Taq (TaKaRa Taq Version 2.0) kit, fungal genomic DNA small purification kit, gene amplification instrument, vertical shaking incubator, UV-Vis spectrophotometer.

[0035] 2. Isolation of strains:

[0036] After processing the collected pineapple rhizosphere soil samples, weigh 10g of soil samples, add 1L of sterile water to mix, shake at 180r / min for 30 minutes, keep the tempera...

Embodiment 2

[0041] Identification of embodiment 2 functional bacteria NJAU-L8

[0042]Strain NJAU-L8 was cultured on PDA medium for 96 hours. The colony grows fast, the hyphae are sparse at the edge, dense in the middle, the colony is yellow-white, the mycelium is loose and densely fluffy, the spores are light yellow, the edges are irregular, opaque, and they are closely connected with the medium and are not easy to be picked; ITS The results of the comparison analysis of the developmental tree constructed by the sequence showed that the strain NJAU-L8 had the highest homology with Talaromyces pinophilus, and the farthest homology with Talaromyces sp. Strain NJAU-L8 was identified as Talaromyces sp. ( figure 1 ). The strain is harmless to crops and non-pathogenic to humans and animals.

Embodiment 3

[0043] Embodiment 3 Functional bacterium NJAU-L8 and the plate antagonism of watermelon wilt pathogen

[0044] The strain NJAU-L8 and the watermelon wilt pathogen were inoculated into PDA medium at the same time, and cultured in a constant temperature incubator at 28°C for 4 days. After the two strains grew well, compared with the PDA plate inoculated with only pathogenic bacteria under the same conditions, it was observed that the strain could significantly inhibit the growth of Fusarium oxysporum watermelon-specific Fon on the PDA medium. The results showed that the functional bacterium NJAU-L8 had an inhibitory effect on the pathogenic bacteria of Fusarium wilt of watermelon ( figure 2 ).

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Abstract

The invention provides a talaromyces pinophilus NJAU-L8 for preventing and controlling continuous crop rotation soil-borne wilt disease and use thereof. The strain has a preservation number of CGMCC No. 21476. The strain is used for reducing number of pathogens in watermelon and banana fusarium wilt planting soil, promoting plant growth and reducing morbidity. It is proved that the strain NJAU-L8 separated from rhizosphere of crop-rotation pineapple can effectively reduce colonization of fusarium oxysporum in rhizosphere of various crops and shows that the strain NJAU-L8 has potential of preventing and controlling wilt disease in actual production.

Description

technical field [0001] The invention belongs to the technical field of biological prevention and control, and in particular relates to a Talaromyces pinophilus NJAU-L8 used for the prevention and control of crop wilts such as bananas and watermelons and its application. Background technique [0002] Soil-borne Fusarium wilt is a worldwide soil-borne disease caused by Fusarium oxysporum, which can occur throughout the growth period. Different specialized pathogens of the same Fusarium oxysporum can cause Fusarium wilt of different crops. Fusarium wilt on watermelon caused by Fusarium oxysporum specialized in watermelon infection and Fusarium wilt on bananas caused by Fusarium oxysporum specialized infection in watermelon have become It is one of the diseases that cause the most serious damage to the planting industry and cause the largest economic loss. Because Fusarium oxysporum can survive in the soil for a long time, once infected, it can be continuously enriched in the s...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00A01P21/00C12R1/645
CPCC12N1/14A01N63/30
Inventor 沈其荣李荣李俞婵沈标袁先福王心怡
Owner NANJING AGRICULTURAL UNIVERSITY