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Adipose-derived stem cell growth factor extract as well as preparation method and application thereof
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A technology of fat stem cells and growth factors, which is applied in the field of fat stem cell growth factor extracts and its preparation, and can solve problems affecting the appearance of the skin
Pending Publication Date: 2022-01-21
维他利肤(北京)生物科技有限公司
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However, this therapy is invasive and carries some risks, and will cause many traumatic wounds that can affect the appearance of the skin
In addition, since living cells are used in this therapy, the safety of living cells should be strictly evaluated before injection, because living cells should be derived from human stem cells, and the application of this traditional cell therapy injected into the human body has many limitations
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[0038] See figure 1As shown, the preparation method of the cell growth factor extract of the present embodiment includes:
[0039] After disinfecting the container of 25 ml of adipose tissue, the adipose tissue was cleaned with a physiological saline, and the upper layer was removed, and the upper layer was removed, and the underlying liquid was cleaved, and the lower layer clean adipose tissue was removed.
[0040] 5 ml of 0.25% collagenase was added to adipose tissue at 37 ° C, 400 g / h, digestion for 30 min.
[0041] Then, the serum-containing MEM medium was added to terminate digestion, and the cells in the digestion were added to the centrifuge tube, 1800 rpm centrifuge for 10min, remove the supernatant, collect the lower cell, MEM medium for 10% fetal bovine serum The dispersion is dispersed evenly, then a cell sieve of 100 μm to obtain a primary fat stem cell precipitate.
[0054] The growth factor-containing supernatant was lyophilized at a temperature of 0.2 Mbar and -30 ° C, and a sterile lyophilized powder was made of 0.13 mg per frozen powder.
[0055] The lyophilized powder was dissolved with a solvent before use, and then the microcrystalline was introduced into the skin, and the growth factor can easily penetrate into the skin, promoting hyaluronic acid, elastin, collagen or meringin in the skin in the skin. Expression.
[0056] Depending on the need to use the object, the lyophilized powder of the adipose stem cell growth factor of the present invention can be used in various application frequencies, for example, 2, once every two weeks. The dose can be adjusted according to the degree of skin aging, and can be increased by 2-5 times.
[0057] figure 2 Use the present invention to use the invention, 2 bonsing each time, once every two weeks. After a month, the freckles have a significan...
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Abstract
The invention discloses a method for preparing adipose-derived stemcell growth factors. The method comprises the following steps: separating adipose-derived stem cells, culturing the adipose-derived stem cells and culturing the adipose-derived stem cell growth factors. The adipose-derived stem cellgrowth factor obtained by the invention can be used for repairing skin aging, especially promoting expression of hyaluronic acid, elastin, collagen and / or reticulateprotein in skin, stimulating cell proliferation of a use area, stimulating skincell proliferation and using the skin area to secrete nutritional factors. The adipose-derived stem cellgrowth factor disclosed by the invention can be used for preparing cosmetics or medical cosmetology products.
Description
Technical field [0001] The invention belongs to the biological field, and more particularly to an adipose stem cell growth factor extract and a preparation method thereof. Background technique [0002] Stem cells are cell populations having self-renewal, high proliferation, and multi-pendant differentiation. Different stem cells can be divided into embryonic stem cells and adult stem cells, embryonic stem cells can differentiate into specific functional stem cells depending on the order of individual development. Such as skin stem cells can be divided into a variety of skin cells, hematopoietic stem cells are differentiated into red blood cells, leukocytes and platelets. Stem stem cells are unlimited stem cells extracted from have differentiated tissues, which can be self-renewed and can differentiate into cells of such tissues under certain conditions. They are mainly used to maintain the steady state of cell function. As the study progressed, it was found that the type of tissu...
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