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Method of treating solid tumors using crocetin

A technology of crocus acid and a treatment method, which is applied in the directions of antitumor drugs, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve problems such as unmet medical needs and the like

Active Publication Date: 2022-01-28
杭州梦岚瑞思生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] There remains an unmet medical need to develop novel therapeutic agents and / or sensitizers for radiotherapy and chemotherapy in the treatment of solid tumors, especially glioblastoma

Method used

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  • Method of treating solid tumors using crocetin
  • Method of treating solid tumors using crocetin
  • Method of treating solid tumors using crocetin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] The preparation of embodiment 1 crocetin drug-loaded liposome preparation

[0086] Weigh 13 mg of lecithin, 5 mg of cholesterol, 2 mg of distearoylphosphatidylethanolamine-polyethylene glycol (PEG-DSPE2000), dissolve in 2 ml of chloroform, add 3 mg of drug (dissolved in DMSO), and place in a film-forming eggplant-shaped The chloroform organic solvent was recovered under reduced pressure in the flask to form a film. Take 2ml of deionized water and add it into an eggplant-shaped flask to rotate and hydrate to obtain drug-loaded liposomes.

Embodiment 2

[0087] Example 2 The inhibitory effect of crocetin combined with radiotherapy on BALB / c nude mouse solid tumor model

[0088] 1. Experimental materials

[0089]Hela human cervical cancer cell line, HCT116 human colon cancer cell line, HepG2 human liver cancer cell line, (ATCC).

[0090] Crocetin acid: brick red powder, HPLC detection purity greater than 98% (Shaoxing Tiankang Biotechnology Co., Ltd.).

[0091] The liposome preparation prepared in Example 1 was used for intravenous injection of crocetin, and 0.5% sodium carboxymethylcellulose was used to prepare crocetin suspension with a certain concentration for intragastric administration.

[0092] TSC: According to the patented method (US6060511), it is prepared with crocetin, and the purity detected by HPLC is greater than 98%.

[0093] Male BALB / c nude mice aged 4-8 weeks were provided by the Experimental Animal Center of Zhejiang Academy of Medical Sciences.

[0094] 2. Method

[0095] 2.1 Modeling

[0096] Take wel...

Embodiment 3

[0113] Example 3 Inhibitory effect of crocetin combined with temozolomide on C6 orthotopic glioma model in BALB / c nude mice

[0114] 1. Experimental materials

[0115] C6 glioma cell line (ATCC).

[0116] Crocetin acid: brick red powder, HPLC detection purity greater than 98% (Shaoxing Tiankang Biotechnology Co., Ltd.).

[0117] The liposome preparation prepared in Example 1 was used for intravenous injection of crocetin, and 0.5% sodium carboxymethylcellulose was used to prepare crocetin suspension with a certain concentration for intragastric administration.

[0118] TSC: According to the patented method (US6060511), it is prepared with crocetin, and the purity detected by HPLC is greater than 98%.

[0119] Temozolomide, the purity is greater than 99% (Guangzhou Tangmushi Life Technology Co., Ltd.).

[0120] Male BALB / c nude mice aged 4-8 weeks were provided by the Experimental Animal Center of Zhejiang Academy of Medical Sciences.

[0121] 2. Method

[0122] 2.1 Modeli...

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Abstract

The invention discloses a novel application of crocetin. In particular, the present disclosure relates to methods of treating solid tumors, such as glioblastoma, by administering to a mammal a pharmaceutically effective amount of crocetin before, during, or after as radiation therapy, chemotherapy, immunotherapy, or a combination therapy thereof. The present disclosure also relates to the use of crocetin in a composition for the treatment of solid tumors, such as glioblastoma.

Description

technical field [0001] The present disclosure relates to crocetin having therapeutic use in the treatment of solid tumors. For example, the present disclosure relates to methods of treating solid tumors, particularly glioblastoma, by administering to a mammal in need thereof a pharmaceutically effective amount of Crocetin. The present disclosure also relates to the use of crocetin in a composition for the treatment of solid tumors, especially glioblastoma. Background technique [0002] Malignant cancers such as hematological malignancies and solid tumors are a serious threat to human health. Treatment for cancer has traditionally been accomplished with one or a combination of surgery, chemotherapy, radiation therapy, immunotherapy, and hormone therapy. With the emergence of new anti-cancer drugs, especially the rise of immunotherapy, the curative effect of blood cancer has been greatly improved in recent years, while radiotherapy and chemotherapy are still the main treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/202A61P35/00
CPCA61K31/202A61P35/00A61K31/495A61K2300/00
Inventor 钱江曹浪
Owner 杭州梦岚瑞思生物科技有限公司