Nucleoside prodrug and application thereof
A kind of use and pharmaceutical technology, applied in the field of medicine, can solve the problems of complex contact environment, etc., and achieve the effect of superior performance, good oral bioavailability, and high plasma drug exposure
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[0088] The present invention will be more readily understood by reference to the following examples, which are presented for illustration and should not be construed as limiting the scope of the invention in any way.
[0089] Unless otherwise defined or clearly dictated by context, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be understood that any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. Unless otherwise stated, the materials and apparatus used in this application were all routinely obtained commercially.
preparation example
[0091] Synthesis of compound 1
[0092]
[0093] Compound 1-1 (4g, 13.7mmol, 1eq) was dispersed in acetone (80mL), and 2,2-dimethoxypropane (7.15g, 68.7mmol, 5eq) and p-toluenesulfonic acid hydrate (2.87g, 15.1 mmol, 1.1 eq). The mixture was stirred overnight at room temperature, then most of the solvent was spun off. The pH of the residue was adjusted to neutral with aqueous sodium bicarbonate, and the mixture was extracted with dichloromethane. The organic phase was dried and spin-dried, and the residue was subjected to silica gel column chromatography (MeOH / DCM=0%-7%) to obtain compound 1-2 (4.41 g, yield 95%).
[0094]Compound 1-2 (300mg, 0.9mmol, 1eq) was dispersed in dichloromethane (20mL), followed by adding isovaleric acid (101.7mg, 0.99mmol, 1.1eq), DMAP (5.5mg, 0.045mmol, 0.05eq) and DCC (224.2 mg, 1.09 mmol, 1.1 eq). The mixture was stirred overnight at room temperature, then filtered. After the filtrate was spin-dried, the residue was subjected to silica ge...
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