Trollius ledebourii flavonoid component extraction process optimization method

A lotus flavonoids, extraction technology, applied to plant/algae/fungi/moss components, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of low melting point and high volatility

Active Publication Date: 2022-02-18
INNER MONGOLIA UNIVERSITY
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Problems solved by technology

Due to the existence of a large number of hydrogen bonds, the melting point of NADESs is extremely low, and the raw material is a green and non-toxic reagent, which overcomes the shortcomings of traditional organic reagents such as high volatility and high toxicity

Method used

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  • Trollius ledebourii flavonoid component extraction process optimization method
  • Trollius ledebourii flavonoid component extraction process optimization method
  • Trollius ledebourii flavonoid component extraction process optimization method

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Embodiment Construction

[0038] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0039] Throughout the specification, unless otherwise specified, terms used herein should be understood as commonly used in the art. Therefore, unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, this specification shall take precedence.

[0040] see Figure 1 to Figure 7 . This embodiment provides a response surface optimization method for the extraction process of flavonoids from Trollius brevifolia, including the following steps:

[0041] (1...

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Abstract

The invention discloses a Trollius ledebourii flavonoid component extraction process optimization method, which comprises the following steps: preparing a natural eutectic solvent, extracting flavonoid substances, performing HPLC (High Performance Liquid Chromatography) analysis, screening the natural eutectic solvent, screening the central level of a main effect factor, performing experimental design and optimization and the like, and finally acquiring a response surface diagram of a regression equation, thus obtaining the optimal extraction condition of the extraction rate of the flavonoid component. The Trollius ledebourii flavonoid component extraction process response surface optimization method provided by the invention has the advantages that main influence factors of the extraction rate are subjected to multi-factor optimization, the defects that a single-factor test is large in number of times, long in period, large in workload, lack of interaction among the factors and the like are overcome, the optimal process conditions are obtained, and a foundation is laid for further research of Trollius ledebourii.

Description

technical field [0001] The invention relates to the technical field of extracting Chinese and Mongolian medicines, in particular to a process optimization method for extracting flavonoid components from Trollus brevipetalum. Background technique [0002] Trollius ledebouri Reichb. is a perennial herb of the family Ranunculaceae, mainly distributed in northeast Inner Mongolia and Heilongjiang. Nasturtium short petals is rich in bioactive substances such as flavonoids, organic acids and sterols, among which flavonoids are its main components. Orientin and vitexin, as flavonoids, are the index active substances of nasturtium brevipetalum. Modern pharmacological studies have shown that orientin has anti-inflammatory, antibacterial, antioxidant and antiviral effects, and vitexin has shown an important role in the treatment of inflammation, cancer and viruses. The anti-oxidative effect and protection of cardiovascular function of nasturtium short petals are also widely used in c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61P31/04A61P29/00A61P39/06A61P35/00A61P31/12A61K133/00
CPCA61K36/71A61P31/04A61P29/00A61P39/06A61P35/00A61P31/12A61K2236/333
Inventor 张艳玲左佳乐董阿力德尔图吴海霞耿淑琴马佩荣李政
Owner INNER MONGOLIA UNIVERSITY
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