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Linear structured light chromatography method, system and device based on strip intensity estimation

A linear structure and intensity technology, applied in the field of optical imaging, can solve problems such as poor image signal-to-noise ratio, weak signal distortion, etc.

Pending Publication Date: 2022-02-18
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the above-mentioned technical deficiencies, and propose a linear structured light tomography method, imaging system and three-dimensional imaging device based on strip intensity estimation, to solve the problem of weak signal distortion and image signal loss in the existing structured light microscopic imaging technology. Problem with poor noise ratio

Method used

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  • Linear structured light chromatography method, system and device based on strip intensity estimation
  • Linear structured light chromatography method, system and device based on strip intensity estimation
  • Linear structured light chromatography method, system and device based on strip intensity estimation

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Effect test

Embodiment 1

[0072] A camera with a line array number N=6 is used to take pictures of the sample, that is, a single frame image includes an image L composed of 6 rows of pixels 1 ~ L 6 . In the case of symmetrical light intensity distribution, L 3 or L 4 For the two line arrays with the strongest light intensity, you can choose L 3 or L 4 Any one of them, choose L below 3 As an example, the signal region template t(x,y) can be expressed as:

[0073]

[0074] Among them, the threshold T is calculated from the captured images using the maximum inter-class variance method in the prior art. In order to obtain a structured light modulation image with uniform phase distribution, 6 images with different phases are generated through virtual digital modulation, and 6 images with different phases The digital modulation function of The following conditions:

[0075]

[0076] Within the range of camera line array number N=6, 6 digital modulation patterns with different phases The valu...

Embodiment 2

[0091] A camera with a line array number N=5 is used to take pictures of the sample, that is, a single frame image includes an image L composed of 5 rows of pixels 1 ~ L 5 . In the case of symmetrical light intensity distribution, L 3 are the two linear arrays with the strongest light intensity, therefore, the signal area template t(x,y) can be expressed as:

[0092]

[0093] Among them, the threshold T is calculated from the captured images using the maximum inter-class variance method in the prior art. In order to obtain a structured light modulation image with a uniform phase distribution, 5 images with different phases are generated through virtual digital modulation, and 5 images with different phases digital modulation function The following conditions:

[0094]

[0095] Within the range of camera line number N=5, 5 digital modulation patterns with different phases The values ​​are: 10000, 01000, 00100, 00010, 00001, that is, the five different phase images ...

Embodiment 3

[0109] The present invention also provides a linear structured light tomography three-dimensional imaging device based on strip intensity estimation, including the above-mentioned linear structured light tomography system based on strip intensity estimation, and also includes:

[0110] The object stage is used to place the object to be imaged;

[0111] The cutting module is used to cut off the shallow layer of the imaged object, and the sample is part of the shallow layer of the object;

[0112] The cutting module moves relatively to the imaging object, so that the cutting module cuts off the shallow layer of the imaged object. Regardless of the movement of the cutting module or the movement of the imaging object, cutting can be realized as long as the two move relatively.

[0113] There is a big difference between three-dimensional object imaging and thin-section imaging. To image three-dimensional objects, it is necessary to demodulate the shallow layer using the above-ment...

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Abstract

The invention provides a linear structured light tomography method based on stripe intensity estimation, under the condition of online illumination imaging, N-array imaging is carried out on a certain sampling area to obtain N sampling images, a reference signal area in a stripe image is obtained through a threshold segmentation method; then, a mathematical modulation pattern is loaded on the camera to obtain N modulation images with different phases, the modulation intensity of each modulation image is estimated within the range of the reference signal area, and finally, an optical tomography image is obtained through linear proportion subtraction. According to the method, the problems of weak signal distortion and signal-to-noise ratio deterioration in traditional nonlinear structured light tomography image reconstruction are avoided, and meanwhile, the method is relatively simple to implement and relatively good in algorithm robustness.

Description

technical field [0001] The invention relates to optical imaging technology, in particular to a linear structured light tomography imaging method based on band intensity estimation, an imaging system and a three-dimensional imaging device. Background technique [0002] High-throughput optical tomography is of great significance for biomedical research. Traditional wide-field microscopy has strong background fluorescence in thick tissue imaging. To address this issue, several technical approaches have been proposed, including confocal microscopy, two-photon microscopy, light-sheet microscopy, and structured light microscopy, among others. Confocal microscopy imaging technology places a small hole in front of the camera to block the out-of-focus background signal, allowing only the focal plane image to pass through, thereby achieving the optical tomography effect. The two-photon microscopy imaging technology utilizes nonlinear effects, and only the focal position has sufficien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T11/00G01N21/84G01N21/01
CPCG06T11/003G01N21/01G01N21/84
Inventor 袁菁姜辰宇金锐龚辉
Owner HUAZHONG UNIV OF SCI & TECH