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Gelatin and nano calcium carbonate copolymerized capillary tube bundle for 3D culture of stem cells and preparation method of gelatin and nano calcium carbonate copolymerized capillary tube bundle

A nano-calcium carbonate and capillary bundle technology, applied in the field of stem cell culture, can solve the problems of inability to study the relationship between 3D proliferation of pore stem cells and unpredictable effects.

Pending Publication Date: 2022-02-22
华子昂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the pores in these porous carriers are randomly generated, either intermittent or continuous, and the pores may be wide or narrow. Cultivate a role in industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of collagen carrier for 3D culture of capillary stem cells

[0025] S1 Mix gelatin and nano-calcium carbonate in proportion (1:0.5) to make glue;

[0026] S2 Draw the glue solution into a capillary with a specific inner diameter of 0.3mm, and use 10% high-concentration glutaraldehyde for rapid fixation;

[0027] S3 combines 7 capillaries and wraps glue on the outer layer to form a capillary bundle, and uses 2.5% glutaraldehyde for fixing and shaping;

[0028] S4 freeze-cutting the shaped capillary bundle to obtain a small section of 10 mm capillary bundle;

[0029] S5 capillary bundles were cleaned to remove glutaraldehyde, dried and dehydrated at low temperature, tested and stored after aseptic treatment, and gelatin and nano-calcium carbonate copolymerized capillary bundles for 3D stem cell culture were obtained.

[0030] (2) 3D culture of MSC stem cells with collagen carrier for 3D culture of stem cells

[0031] S6 uses the stem cell 3D culture ins...

Embodiment 2

[0035] (1) Preparation of collagen carrier for 3D culture of capillary stem cells

[0036] S1 Mix gelatin and nano-calcium carbonate in proportion (1:1) to make glue;

[0037] S2 Draw the glue solution into a capillary with a specific inner diameter of 0.1mm, and use 10% high-concentration glutaraldehyde for rapid fixation;

[0038] S3 combines 6 capillaries and wraps glue on the outer layer to form a capillary bundle, and uses 2.5% glutaraldehyde for fixing and shaping;

[0039] S4 freeze-cutting the shaped capillary bundle to obtain a small section of 5mm capillary bundle;

[0040] S5 capillary bundles were cleaned to remove glutaraldehyde, dried and dehydrated at low temperature, tested and stored after aseptic treatment, and gelatin and nano-calcium carbonate copolymerized capillary bundles for 3D stem cell culture were obtained.

[0041] (2) 3D culture of MSC stem cells with collagen carrier for 3D culture of stem cells

[0042] S6 uses the stem cell 3D culture instrum...

Embodiment 3

[0046] (1) Preparation of collagen carrier for 3D culture of capillary stem cells

[0047] S1 Mix gelatin with nano-calcium carbonate and lysine in a ratio of (1:2:0.5) to make a glue;

[0048] S2 Draw the glue solution into a capillary with a specific inner diameter of 2mm, and use 10% high-concentration glutaraldehyde for rapid fixation;

[0049] S3 combines 3 capillaries and wraps glue on the outer layer to form a capillary bundle, and uses 2.5% glutaraldehyde for fixing and shaping;

[0050] S4 freeze-cutting the shaped capillary bundle to obtain a 20 mm small section of the capillary bundle;

[0051] S5 capillary bundles were cleaned to remove glutaraldehyde, dried and dehydrated at low temperature, tested and stored after aseptic treatment, and gelatin and nano-calcium carbonate copolymerized capillary bundles for 3D stem cell culture were obtained.

[0052] (2) 3D culture of MSC stem cells with collagen carrier for 3D culture of stem cells

[0053] S6 uses the stem cel...

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Abstract

The invention provides a gelatin and nano calcium carbonate copolymerized capillary tube bundle for 3D culture of stem cells and a preparation method thereof. The preparation method comprises the following steps: mixing gelatin and nano calcium carbonate to prepare a glue solution, drawing the glue solution containing nano calcium carbonate into a capillary tube with a specific inner diameter, and carrying out rapid surface fixation by using glutaraldehyde; and wrapping the outer layers of a plurality of capillary tubes with the glue solution to prepare a capillary tube bundle, placing the capillary tube bundle in a glutaraldehyde solution to complete fixation and shaping, freezing and cutting the shaped capillary tube bundle to form small sections, cleaning and removing glutaraldehyde, and performing low-temperature drying and sterile treatment to obtain the gelatin and nano calcium carbonate copolymerized capillary tube bundle for 3D culture of stem cells. And stem cells can be cultured in the capillary tube with a specific aperture.

Description

technical field [0001] The invention relates to the field of stem cell culture, in particular to a gelatin-nano-calcium carbonate copolymerized capillary bundle for 3D stem cell culture and a preparation method thereof. Background technique [0002] There are many types of stem cells, which have been widely used in many fields such as disease treatment, medical beauty, anti-aging, and drug screening. Stem cell culture has become a bottleneck restricting the development of the entire stem cell industry chain, and stem cell incubators have become a development hotspot in the field of stem cell culture. According to the characteristics of stem cell culture devices, it can be divided into two categories: 2D culture and 3D culture. 2D culture is mainly carried out by enlarging the dish, changing liquid in batches, and supplying gas by diffusion method. 3D culture is mainly completed in a three-dimensional container, which can independently control the composition of the culture...

Claims

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Application Information

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IPC IPC(8): C12N5/00C12N5/0775
CPCC12N5/0075C12N5/0062C12N5/0662C12N2513/00C12N2531/00C12N2533/54C12N2533/20
Inventor 华子昂刘宝全朱美瑛万君兴竹添张建赵凯龙
Owner 华子昂
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