Method for constructing electrochemical luminescence system by using luminescent compound

Abstract

The invention discloses a method for constructing an electrochemical luminescence system by using a luminescent compound, which specifically comprises the following steps: treating a bare glassy carbon electrode, thoroughly drying, dissolving an electrochemical luminescence reagent pentaaryl substituted cyclopentadienol compound and a co-reactant K2S2O8 in a phosphate buffer solution, taking the glassy carbon electrode as a working electrode, and carrying out electrochemical luminescence by using the glassy carbon electrode as the working electrode. The platinum electrode and the Ag/AgCl electrode are respectively used as a counter electrode and a reference electrode to be immersed in a water phase, the electrochemical luminescence system is easy to construct, the dosage of the used luminescence agent is small, the generated ECL signal is strong, and the electrochemical luminescence system has good stability and reproducibility, and when the water content of the luminescence molecule in a THF/H2O mixed solvent is increased from 0% to 90%, the electrochemical luminescence system has good electrochemical luminescence effect. Compared with a 9, 10-diphenylanthracene (DPA) illuminant, the 9, 10-diphenylanthracene illuminant has the advantages that the fluorescence emission spectrum and ECL emission are gradually enhanced, the ECL emission is enhanced by 4 times compared with the 9, 10-diphenylanthracene (DPA) illuminant, and the luminous efficiency and the sensitivity are obviously improved.

Description

technical field [0001] The invention belongs to the technical field of building an electrochemiluminescence system, and in particular relates to a method for building an electrochemiluminescence system using a luminescent compound. Background technique [0002] Most of the earliest studies on the electrochemiluminescent system were some condensed ring aromatic hydrocarbons, such as pyrene compounds, indoles, anthracenes, and some other light emitters, such as porphyrin compounds. [0003] However, since most organic compounds are hydrophobic planar molecules, they are poorly soluble in aqueous systems and prone to π-π stacking, resulting in fluorescence quenching, which greatly weakens their fluorescence intensities in the solid state and in aggregated states. To a certain extent, the application of organic light emitters in aqueous electrochemiluminescent systems is limited. [0004] The emission rate in dilute solution is low, and the emission rate of aggregated state (in...

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Problems solved by technology

[0003] However, since most organic compounds are hydrophobic planar molecules, they are poorly soluble in aqueous systems and prone to π-π stacking, resulting in fluorescence quenching, which greatly weakens their fluorescence intensities in the solid state and in aggregated states. To a certain extent, the application of organic light emitters in aqueous electrochemiluminescent systems is limited

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