Compositions for prostaglandin transporter inhibition and related therapeutic applications
A prostaglandin and transporter technology, applied in the field of compositions for inhibiting prostaglandin transporters, can solve the problems of no reported inhibition or blocking
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Embodiment 1
[0038] Example 1: Details of the composition
[0039] The present invention discloses a composition comprising 30-60% w / w garlic extract, 10-30% w / w beet extract, 5-15% w / w nigella extract and 10- 30% w / w Terminalia arjuna extract for inhibition of PGT. The above extracts have been reported to elicit cardioprotective and antihypertensive effects individually. Some of the medicinal properties of the above extracts are mentioned below:
[0040] Garlic is a well known plant which is used in the traditional Ayurveda system. It has been reported to elicit many therapeutic effects against different diseases, including cardiovascular diseases, regulate blood pressure, and lower blood sugar and cholesterol levels. It is also effective against bacterial, viral, fungal, and parasitic infections and reportedly boosts the immune system. The plant also has antitumor properties (Ayaz and Alpsoy, Garlic (Allium sativum) and traditional medicine, Turkiye Parazitol Derg. 2007; 31(2):145-9)...
Embodiment 2
[0047] Example 2: Inhibition of Prostaglandin Transporter
[0048]The formulation was evaluated for its ability to inhibit the prostaglandin transporter (PGT). The kidney cell line MDCK was seeded on six-well plates at 30% confluency. Three days later, they were treated with 10 [mu]M bradykinin (to increase endogenous PGE2 synthesis, available at Sigma-Aldrich) at 37[deg.] C. for different durations in the presence of vehicle (DMSO). The formulations were added simultaneously to the cell culture in graded concentrations and incubated for a further 24 hours. Culture medium was collected for measurement of extracellular PGE2 concentration. Cells were washed twice with phosphate-buffered saline, lysed with 250 μl of phosphate-buffered saline containing 0.1 M HCl and 0.1% Triton X-100 for 15 min at room temperature, and then scraped off the plate. Pipette the cell suspension up and down several times to ensure complete lysis. Cell lysates were collected and centrifuged at 10,0...
Embodiment 3
[0053] Embodiment 3: the antihypertensive effect of preparation
[0054] method
[0055] Normotensive Wistar rats were used for the experiments. By administering DOCA (deoxycorticosterone acetate), a synthetic mineralocorticoid derivative, 25 mg / kg s.c, twice a week and 1% w / v NaCl in drinking water, for a period of 30 days (model: DOCA in rats Salt-induced hypertension) spontaneously induces hypertension in rats. Rats were divided into the following groups, each containing 6 rats:
[0056] Table 2: Grouping
[0057] Group describe I normal control II salt loaded III 50 mg / kg body weight formulation IV 100 mg / kg body weight formulation V 200 mg / kg body weight formulation VI 400mg / kg body weight formulation
[0058] The following parameters were estimated to evaluate the antihypertensive effect of the formulation
[0059] · PGT expression
[0060] · Nitric Oxide Estimation
[0061] ·blood pressure
[0062] · Heart ...
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