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Effect of HOXC10 in gastric cancer pathogenesis

A technology of HOXC10 and gastric cancer cells, applied in the field of gastric cancer, can solve the problems of biological function, molecular mechanism and lack of epigenetics

Pending Publication Date: 2022-03-04
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the research on the biological function, molecular mechanism and epigenetics of HOXC10 in the malignant development of gastric cancer is still relatively lacking.

Method used

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  • Effect of HOXC10 in gastric cancer pathogenesis
  • Effect of HOXC10 in gastric cancer pathogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0004] 1. In vitro culture of cell lines (Cell Culture)

[0005] 1.1. Related reagents and instruments

[0006] 1.1.1. Reagents

[0007] DMEM, RPMI 1640, KSFM, trypsin, FBS, PBS.

[0008] 1.1.2. Cultivation related instruments and consumables

[0009] Biological safety cabinets, carbon dioxide incubators, inverted phase contrast microscopes, cell culture flasks, cell culture dishes, cell culture plates, cryotubes, pipette tips.

[0010] 1.2. Method

[0011] Normal human gastric mucosal cells GES-1 were cultured in KSFM medium containing 10% FBS at 37°C, 5% CO 2 Gastric cancer cell lines AGS, MKN28, NCI-N87, and MGC803 were cultured in RPMI 1640 medium containing 10% FBS at 37°C and 5% CO 2 Gastric cancer cell lines HGC27, MKN45, and SGC7901 were all cultured in DMEM medium containing 10% FBS at 37°C, 5% CO 2 cultured in an incubator. The changes in cell morphology were observed every day, and the cell medium was changed every other day. After the cell density reached 85...

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Abstract

The invention relates to a potential cancer promoting gene (HOXC10) highly expressed in gastric cancer, which can promote invasion and metastasis of gastric cancer cells. Four pairs of shRNAs and recombinant lentiviruses are invented, can be chemically synthesized, have high HOXC10 silencing expression efficiency and have a potential treatment effect on gastric cancer.

Description

technical field [0001] The invention herein relates to the role of gene expression in the pathogenesis of cancer, in particular gastric cancer. Background technique [0002] Gastric cancer (Gastric Cancer) is one of the malignant tumors with the highest incidence in the world. According to statistics, in 2015, the number of new cases of gastric cancer in my country was about 680,000, and the number of deaths was about 500,000, ranking second among malignant tumors. Second only to lung cancer in women and breast cancer in women. Surgical treatment is still the first choice for gastric cancer. With the improvement of medical technology, the early diagnosis rate under endoscopy has been significantly improved and corresponding treatment can be carried out in time. However, if tumor metastasis occurs, surgery is often difficult and only palliative treatment can be accepted. Homeobox (Homeobox) gene is a regulatory gene family that encodes specific nucleoprotein (Homeoprotein, h...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/867C12Q1/6886G01N33/574
CPCC12N15/113C12N15/86C12Q1/6886G01N33/57484G01N33/57446C12N2310/14C12N2740/15043C12Q2600/158
Inventor 朱森林姚声张译
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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