PEEK material subjected to exosome surface functionalization treatment
A technology of surface functionalization and exosomes, applied in the direction of prosthesis, biochemical equipment and methods, bone/connective tissue cells, etc., can solve problems such as difficult to promote bone formation in vivo
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[0030] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific implementations described here are only used to explain the present invention, not to limit the present invention.
[0031] figure 1 As shown, the exosome surface functionalized PEEK material realized by the present invention is prepared by the following method:
[0032] Step 1, culturing mesenchymal bone stem cells, which are obtained from bilateral humerus and femur of female SD rats;
[0033] Among them, mesenchymal stem cells were cultured in Dulbecco's modified eagle medium Dulbecco (DMEM, GIBCO) containing 10% fetal bovine serum (FBS, GIBCO) at 37°C and 5% CO 2 .
[0034] At the same time, RAW264.7 culture was carried out, RAW264.7 cells were purchased from ATCC cell bank, in 1640 medium (GIBCO) at 37 °C and 5% CO 2 to culti...
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