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Method for extracting trilobatin from lithocarpus polystachyus leaves

A kind of technology of Keye polyacrylox and trilobatin, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of expensive high-speed countercurrent chromatography and unsatisfactory yield of trilobatin , to achieve the effect of high product yield, low production cost and high content

Pending Publication Date: 2022-03-11
CHANGZHOU HI TECH DISTRICT MULTIPLE DIMENSION IND TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the patent obtained high-purity trilobatin, expensive high-speed countercurrent chromatography was used, and the yield of trilobatin was not satisfactory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for extracting trilobatin from the leaves of Claceae, the method comprises the steps of:

[0030] (1) Dry and pulverize the leaves of Lithocarpus fruticosa, pass through a 200-mesh sieve, collect the crushed particles, mix 20kg of crushed particles, 7kg of nutrient material, 10kg of water, and 4kg of bacterial powder, and put them in a fermenter to ferment for 3 days. Control the temperature at 35-40°C, collect the fermentation mixture, and add porous granules with a mass of 20% of the crushed granules after 32 hours of fermentation;

[0031] (2) Stir the fermentation mixture and 1.5mol / L ethanol solution at a mass ratio of 8:10 at 300r / min for 30min, press filter, collect the press-filtrate, centrifuge the press-filtrate at 9000r / min, collect the centrifuge, and depressurize Concentrate to 75% of the original volume and collect the concentrate;

[0032] (3) Stir the concentrated solution and 1.5mol / L ethanol solution evenly at a volume ratio of 1:2, heat to 4...

Embodiment 2

[0035] A method for extracting trilobatin from the leaves of Claceae, the method comprises the steps of:

[0036] (1) Dry and pulverize the leaves of Lithocarpus fragrans, pass through a 200-mesh sieve, collect the crushed particles, mix 25kg of crushed particles, 9kg of nutrient material, 15kg of water, and 6kg of bacterial powder evenly, and put them in a fermenter to ferment for 3 days. Control the temperature at 35-40°C, collect the fermentation mixture, and add porous granules with a mass of 30% of the crushed granules after 32 hours of fermentation;

[0037] (2) Stir the fermentation mixture and 1.5mol / L ethanol solution at a mass ratio of 8:15 at 300r / min for 30min, press-filter, collect the press-filtrate, centrifuge the press-filtrate at 9000r / min, collect the centrifugate, and depressurize Concentrate to 75% of the original volume and collect the concentrate;

[0038] (3) Stir the concentrated solution and 1.5mol / L ethanol solution evenly at a volume ratio of 1:4, h...

Embodiment 3

[0041] A method for extracting trilobatin from the leaves of Claceae, the method comprises the steps of:

[0042] (1) Dry and pulverize the leaves of Lithocarpus fragrans, pass through a 200-mesh sieve, collect the crushed particles, mix 22kg of crushed particles, 8kg of nutrients, 13kg of water, and 5kg of bacterial powder, and put them in a fermenter for fermentation for 3 days. Control the temperature at 35-40°C, collect the fermentation mixture, and add porous particles with a mass of crushed particles of 25% after 32 hours of fermentation;

[0043] (2) Stir the fermentation mixture and 1.5mol / L ethanol solution at a mass ratio of 8:13 at 300r / min for 30min, press filter, collect the press-filtrate, centrifuge the press-filtrate at 9000r / min, collect the centrifugate, and depressurize Concentrate to 75% of the original volume and collect the concentrate;

[0044] (3) Stir the concentrated solution and 1.5mol / L ethanol solution evenly at a volume ratio of 1:3, heat to 40°C...

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Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to a method for extracting trilobatin from lithocarpus polystachyus leaves. In the extraction process, porous particles prepared by taking gelatin, activated carbon and polyacrylamide as raw materials are added, substances released in the fermentation process can be effectively subjected to impurity removal and decoloration through a porous structure, the purity of the product in the fermentation process is improved, the purity of trilobatin and the yield of the product are improved, and the yield of the trilobatin is improved. According to the extraction method, the lithocarpus polystachyus leaves can be effectively extracted, the lithocarpus polystachyus leaves have high-content active ingredients, the product yield is high, the product content is high, the equipment requirement is low, the production cost is low, and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting trilobatin from leaves of Coede polyacrysm. Background technique [0002] Duosuike is an evergreen plant of the genus Shike of the Fagaceae, also known as sweet tea, Duosuike or sweet tea. Studies have found that Duosuishike mainly contains flavonoids, triterpenoids, polyphenols and other ingredients, which have anti-oxidation, blood fat-lowering, blood-sugar-lowering, anti-allergic, antibacterial and other pharmacological effects, and are mostly used in medicine, beauty, food, and health care. products and other fields. Its young leaves have a high sweetness after soaking. Modern research shows that its main sweetness component is dihydrochalcone natural sweetener, especially trilobatin content is the highest. [0003] The natural sweetener is the main sweetness component of the young leaves of Duosuike. It is a natural sweetener wi...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08B01J20/20B01J20/24B01J20/26B01J20/30
CPCC07H15/203C07H1/08B01J20/20B01J20/24B01J20/261B01J2220/46
Inventor 顾书华李庆宜吕伟红
Owner CHANGZHOU HI TECH DISTRICT MULTIPLE DIMENSION IND TECH INST
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