Cell culture device capable of monitoring nasopharynx cancer primary cell culture liquid environment

A primary cell, liquid environment technology, used in tissue cell/virus culture devices, biochemical cleaning devices, enzymology/microbiology devices, etc.

Inactive Publication Date: 2022-03-11
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a cell culture device capable of monitoring the liquid environment for nasopharyngeal carcinoma primary cell culture, which has the beneficial effect of detecting the culture liquid without taking out the culture dish, and solves the problems mentioned in the above-mentioned background technology

Method used

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  • Cell culture device capable of monitoring nasopharynx cancer primary cell culture liquid environment
  • Cell culture device capable of monitoring nasopharynx cancer primary cell culture liquid environment
  • Cell culture device capable of monitoring nasopharynx cancer primary cell culture liquid environment

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Experimental program
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Effect test

Embodiment 1

[0030] See Figure 1-8 A monitor may be primary cell cultures of NPC cell culture apparatus of the liquid environment, comprising an incubator and a plurality of primary cell culture bottle 10, the inside of the incubator 1 is provided with a loading dish assembly, a plurality of original cell culture flask by dish 10 is placed on the inside of the loading assembly incubator 1, the incubator 1 and the inner side below the dish loading assembly is provided with a mounting assembly drain pan, inside incubator 1 via the drainage pan mounted assembly is provided with a plurality of drainage activities dishes, dish drainage installation position number, respectively and a plurality of positions corresponding to the primary cell culture bottle 10, the bottom of the primary cell culture flask provided with a bottom protrusion 10 of the structure 30, the raised bottom structure center of the bottom 30 is provided with a concave bottom structure 25, the bottom of the concave inner side wall...

Embodiment 2

[0033] This embodiment is a modification made on one embodiment embodiment, please refer to specific Figure 5 , Image 6 and Figure 8 , A bottom concave inner side wall structure 25 defines a plurality of chutes 28, outer rubber seal block 31 and the corresponding positions of two chutes 28 are provided with a slider 29, two sliders 29 are connected to card activity two inner side of the chute 28, the bottom of the indentations 25 and the inner side of the rubber seal 31 and a bottom block indentations 25 between the top surface active sleeved inside a first return spring 26, the first return spring 26 for urging rubber seal block 31 is reset, the drainage pan mounting assembly 14 comprises two lateral plates, two horizontal plates 14 are fixedly mounted between the inner sides of the incubator 1, the two lateral plates 14 each defines a number of through holes 15, there is a gap between the two lateral plates 14, the outer diameter of the through hole 44 of the draft tube and the ...

Embodiment 3

[0036] This embodiment is a modification made on one embodiment embodiment, please refer to specific figure 2 , image 3 and Figure 7 , The loading dish comprises a cap assembly support plate 21, bottom plate 27 and the frame number of the bottom support plate 19, the inner bottom surface of the incubator 1 is fixedly mounted with a plurality of stopper posts 18, the support plate 21 and the cap plate 27 frame and the corresponding position on the retaining post 18 are each defines a limiting hole 20, the cap 21 and the frame plate supporting the bottom plate 27 by limiting hole 20 between the socket 18 active, caps several limiting post support plate between the upper surface of the bottom plate 21 of the lower surface of the frame 27 is fixedly mounted with a plurality of posts 40, a plurality of bottom support plates 19 are fixedly mounted to the inner side of the frame bottom plate 27, the upper surface of the cap support plate 21 defines a plurality of the first set of holes 2...

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Abstract

The invention relates to the technical field of medical culture devices, and discloses a cell culture device capable of monitoring nasopharynx cancer primary cell culture liquid environment, the cell culture device comprises a constant temperature culture box and a plurality of primary cell culture flasks, the inner side of the constant temperature culture box is provided with a culture dish loading assembly, and the culture dish loading assembly is provided with a plurality of primary cell culture flasks; the plurality of primary cell culture bottles are placed on the inner side of the constant-temperature incubator through the culture dish loading assembly. According to the cell culture device capable of monitoring the nasopharynx cancer primary cell culture liquid environment, a pushing assembly can enable a drainage vessel to move upwards, so that a jacking column moves upwards, and the jacking column moves upwards to push a rubber sealing block, so that the rubber sealing block does not block a leak hole any more; therefore, a culture solution on the inner side of the primary cell culture bottle flows to the inner side of the concave structure at the bottom through the leak hole and is discharged to the outer side of the constant-temperature incubator along the drainage tube, and the effect that the culture solution on the inner side of the primary cell culture bottle can be detected without taking out the primary cell culture bottle from the inner side of the constant-temperature incubator is achieved.

Description

Technical field [0001] The present invention relates to the technical field of medical culture apparatus, in particular a cell culture apparatus that can monitor the cultivation of nasopharyngeal carcinoma primary cell culture liquid environment. Background technique [0002] Primary culture refers to the first cultivation of tissue or cells in vivo, also called initial culture. The original culture is short, and the genetic traits and in vivo cells are similar, suitable for cell morphology, function and differentiation. More stringent refers to the cultivation before successful passage. At this time, the cells maintain the basic properties of the original cell. If it is normal cell, it still retains the double number, but in fact, usually within the first generation to tenth generation. The cultured cells are collectively referred to as primary cell culture. The most commonly used primary culture has tissue block culture and dispersion cell culture. [0003] Existing primary cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/26C12M1/24C12M1/00B08B9/093
CPCC12M23/08C12M23/38C12M23/48C12M33/04C12M39/00B08B9/093
Inventor 黄宁任艳鑫李池川王婷张黎邱琴褚永双
Owner KUNMING MEDICAL UNIVERSITY
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