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Fluorescence light path system applied to fluorescence imaging

A fluorescent optical path and fluorescent imaging technology, applied in the field of fluorescent optical path systems, can solve the problems of uneven illumination of bright field and illumination fluorescence, light attenuation, etc., so as to reduce the loss of light source, increase the intensity of excitation light, and solve the effect of uneven illumination of light

Pending Publication Date: 2022-03-18
GUANGZHOU BLT INSTR & METER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the above-mentioned existing technical defects, the present invention provides a fluorescence optical path system applied to fluorescence imaging, which solves the problems of illuminating bright field and illuminating fluorescence unevenness and light attenuation, and realizes the purpose of transmitted fluorescence imaging

Method used

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  • Fluorescence light path system applied to fluorescence imaging
  • Fluorescence light path system applied to fluorescence imaging
  • Fluorescence light path system applied to fluorescence imaging

Examples

Experimental program
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Effect test

Embodiment 1

[0064] Such as Figure 1-7 As shown, a fluorescence optical path system applied to fluorescence imaging shown in this embodiment is specifically installed on an imaging instrument 100 for animal imaging, including:

[0065] The object stage 11 is used to place small animals (such as mice) that need to be imaged. It is installed on the Z-axis driving mechanism of the imaging instrument so that it can move up and down along the Z-axis direction, and the middle of the object stage 11 is provided with The detachable adapter 10 is used to match small animals of different sizes by replacing different adapters. There are several light-transmitting holes 12 arranged in an array through the adapter 10. Each small hole can transmit excitation light. The holes can also be used independently; in addition, in a specific embodiment, the light-transmitting holes can be divided into left and right parts, so that two small animals can be respectively placed above a part of the light-transmitti...

Embodiment 2

[0090] Such as Figure 12 and 13 As shown, a fluorescence optical path system applied to fluorescence imaging shown in this embodiment, wherein the structure of the stage, transmission light path, illumination light path and imaging device is the same as that of Embodiment 1, and its difference from Embodiment 1 is It lies in the LED light source module and its matching structure, specifically:

[0091] The plurality of LED light source modules used to emit a single color light source in Embodiment 1 are replaced with two LED light source modules 6 that can be used to emit a full-spectrum light source, and the two LED light source modules 6 are fixed in the imaging instrument for respectively The input end of the first optical fiber light guide / second optical fiber light guide corresponds to the front and rear; specifically, the LED light source module 6 includes a hollow cylinder 61 with an opening at one end, a full-spectrum LED lamp 65 arranged in the hollow cylinder 61, t...

Embodiment 3

[0096] Such as Figure 14 and 15 As shown, a fluorescence optical path system applied to fluorescence imaging shown in this embodiment, wherein the structure of the stage, transmission light path, illumination light path and imaging device is the same as that of Embodiment 1, and its difference from Embodiment 1 is It lies in the LED light source module and its matching structure, specifically:

[0097]Replace the LED light source module in Embodiment 1 with two LED lamps 71 that can emit full-spectrum light sources and a plurality of optical path processing modules 72 that can transmit different light source colors, wherein the two LED lamps 71 are fixed in the imaging instrument and Corresponding to the front and back of the input ends of the first optical fiber light guide and the second optical fiber light guide respectively.

[0098] The optical path processing module 72 includes a hollow cylinder 61 with openings at both ends, a focusing lens 63 disposed in the hollow ...

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Abstract

The invention provides a fluorescence light path system applied to fluorescence imaging, which comprises a plurality of LED light source modules, an imaging device, an objective table, a condensing lens, a first optical fiber light transmitting bundle of which the output end is connected with the condensing lens, a hollow shell and a second optical fiber light transmitting bundle of which the output end is connected with the hollow shell, a through hole is formed in the middle of the hollow shell in a penetrating mode, a plurality of light outlet holes are formed in the bottom face of the hollow shell, the second optical fiber light guide bundle comprises an external sheath and an optical fiber bundle arranged in the sheath, the light outlet end of the optical fiber bundle is divided into a plurality of sub-bundles which are equal to the light outlet holes in number and correspond to the light outlet holes one to one, and the tail ends of the sub-bundles are arranged at the light outlet holes; the colors emitted by the LED light source modules are different, and the different LED light source modules are driven by a driving device to be aligned with the input ends of the first optical fiber light transmitting bundle and the second optical fiber light transmitting bundle front and back. According to the invention, the problems of uneven illumination of bright field and illumination fluorescence and light attenuation are solved, and the purpose of transmission fluorescence imaging is realized.

Description

technical field [0001] The invention relates to the field of imaging instruments, in particular to a fluorescence optical path system applied to fluorescence imaging. Background technique [0002] Animal models are important experimental methods and research means in modern biomedical research, which help to understand and understand the occurrence and development of human diseases more conveniently and effectively, and to develop effective preventive and therapeutic measures based on them. Since humans and mice share approximately 99% of the similar genes, research on small animals such as rats, guinea pigs, and mice is increasing in many fields such as life sciences, medical research, and drug development. In recent years, various imaging technologies have played an increasingly important role in animal research, and a variety of professional equipment for imaging small animals has emerged, providing powerful tools for scientific research. [0003] Live animal imaging tec...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6456G01N2021/6463
Inventor 王卫伟罗文波王振国
Owner GUANGZHOU BLT INSTR & METER