Determination method for active ingredients of Juanbi decoction preparation

A method of determination and technology of active ingredients, applied in the field of determination of active ingredients of Juanbi Decoction preparations, can solve problems such as the inability to comprehensively evaluate and control the quality of Juanbi Decoction, and achieve good detection stability, good stability, and good repeatability Effect

Active Publication Date: 2022-03-18
SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN113325094A discloses a method for simultaneous determination of three active ingredients in Juanbi Granules, an ancient classic famous prescription compound preparation, in which chlorogenic acid, ferulic acid and gentian were detected at 320nm and 277nm by high performance liquid chromatography Picroside, the mobile phase used is acetonitrile-0.1% formic acid water. Obviously, the quality of Juanbi Decoction cannot be fully evaluated and controlled through these three chemical components. It is still a technical problem to be able to simply and accurately determine the multi-component content of Juanbi Decoction
Juanbi Decoction has broad prospects for application. Currently, the research on this formula mainly focuses on clinical application, and there are few related reports on the identification of chemical components and quality control.

Method used

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  • Determination method for active ingredients of Juanbi decoction preparation
  • Determination method for active ingredients of Juanbi decoction preparation
  • Determination method for active ingredients of Juanbi decoction preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of embodiment 1 reference substance solution

[0034] Accurately weigh 7 reference substances of gentiopicroside, ferulic acid, procuridine, cinnamon aldehyde, ammonium glycyrrhizinate, osthole, 11-carbonyl-β-acetyl boswellic acid, add methanol with a mass concentration of 80% Each 1ml solution contains 189.5 μg gentiopicroside, 49.7 μg ferulic acid, 50.42 μg cinnamin, 88.65 μg cinnamic aldehyde, 95.68 μg ammonium glycyrrhizinate, 55.25 μg osthole, and 11-carbonyl-β - A mixed solution of mastic 155.15 μg.

Embodiment 2

[0035] Example 2 Preparation of Juanbi Decoction Granules

[0036] Take 3.73kg notopterygium, 3.73kg duhuo, 1.87kg cinnamon, 3.73kg Gentiana, 11.19kg angelica, 2.61kg Chuanxiong, 1.87kg honey-roasted licorice, 7.46kg Caulis chinensis, 11.19kg mulberry branch, 2.98kg frankincense, 2.98kg woody incense, Add water to decoct twice, add 12 times the amount of water for the first time, decoct for 2 hours, add 10 times the amount of water for the second time, decoct for 1 hour, combine the two extracts, filter with a 200-mesh filter cloth while it is hot, and the filtrate Concentrate under pressure, dry, pulverize, and granulate to obtain Juanbi Tang granules;

Embodiment 3

[0037] The preparation of embodiment 3 need testing solution

[0038] Accurately weigh 10 groups of granules in Example 2, each group of 0.5g, put in a stoppered Erlenmeyer flask, accurately add 25ml of 80% methanol, seal the plug, weigh it, and process it ultrasonically for 30 minutes with a power of 250W and a frequency of 40kHz. Cool, weigh again, make up the lost weight with 80% methanol, shake well, filter, and take the filtrate to get final product;

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Abstract

The invention belongs to the technical field of medicinal preparation and traditional Chinese medicine ingredient detection, and particularly relates to a method for determining active ingredients of a Juanbi decoction preparation. The method comprises the following steps: firstly, preparing Juanbi decoction into granules, adopting an UPLC (Ultra Performance Liquid Chromatography) method, taking acetonitrile-0. 1% phosphoric acid water as a mobile phase, and carrying out gradient elution to simultaneously detect gentiopicroside, ferulic acid, decurtin, cinnamaldehyde, ammonium glycyrrhizinate, cnidium lactone and 11-carbonyl-beta-acetyl masticinic acid in the active ingredients of the Juanbi decoction as a classic famous formula compound preparation; the determination method provided by the invention has the advantages of good stability, good repeatability and good recovery rate, and can be used as a quality control means of the Juanbi decoction preparation.

Description

technical field [0001] The invention belongs to the technical field of detection of pharmaceutical preparations and components of traditional Chinese medicines, and in particular relates to a method for determining the active components of Juanbi Decoction preparations. [0002] technical background [0003] Juanbi Decoction, from the "Medical Mind Enlightenment" written by Cheng Guopeng in the Qing Dynasty, is a common prescription for treating wind-cold-damp arthralgia clinically. The prescription is composed of 11 herbs including Notopterygium, Duhuo, Guixin, Gentiana chinensis, Angelica, Chuanxiong, Honey-roasted Glycyrrhiza, Caulis Caulis, Mori, Frankincense, and Woody. One of the 100 classic famous prescriptions formulated by the Administration Bureau in the "Catalogue of Ancient Classical Famous Prescriptions (First Batch)". [0004] "The three qi of wind, cold, and dampness form numbness", which means that exogenous evils such as wind, cold, and dampness invade the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/60
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/6052Y02A50/30
Inventor 曾杉林碧珊高永坚陈锦霞陈伟彦陈伟钢张汝莹汤春花
Owner SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD
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