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Paeonia suffruticosa fermentation stock solution as well as preparation method and application thereof

A technology of fermented liquid and peony, applied in the field of fermented peony liquid and its preparation, can solve the problems of cumbersome operation steps, strict Chinese conditions, danger, etc., and achieve good application prospects, reduce losses, and good anti-oxidation effects

Active Publication Date: 2022-03-22
美出莱(杭州)化妆品有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, organic solvents are often used to extract the active ingredients in the peony plant, which not only causes environmental pollution, but also has high extraction costs. There may be residual organic solvents in the extract, which is not conducive to applying to cosmetic raw materials, and the experimental operation has certain limitations. Dangerous, Chinese-style conditions are strict, not conducive to industrialization
In addition, the extraction efficiency of a certain active ingredient of peony in the existing technology is low, the extraction time is long, and the operation steps are cumbersome

Method used

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  • Paeonia suffruticosa fermentation stock solution as well as preparation method and application thereof
  • Paeonia suffruticosa fermentation stock solution as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0041] The preparation method of the peony fermentation stoste described in the present embodiment, comprises the steps:

[0042](1) Mix 6 g of powdered peony and 300 mL of deionized water evenly, sterilize in an autoclave at 121° C. for 20 minutes after mixing evenly, and cool to room temperature after sterilization to obtain a mixed solution;

[0043] (2) Insert lactic acid bacteria into the mixed solution for fermentation, wherein the lactic acid bacteria are Lactobacillus plantarum and Lactobacillus bulgaricus, and the number of viable bacteria in the lactic acid bacteria liquid is 10 10 CFU / mL, based on the volume of deionized water (300mL) in the initial fermentation system, the inoculum size of lactic acid bacteria is 0.4vol%, and the ratio of viable bacteria of the Lactobacillus plantarum and Lactobacillus bulgaricus is 1:1, at 43°C Cultivate in a constant temperature oscillator for 16 hours, and the shaking table speed is 180r / min; after the fermentation is completed,...

Embodiment 2

[0046] The preparation method of the peony fermentation stoste described in the present embodiment, comprises the steps:

[0047] (1) Mix 6g of powdered peony and 120mL of deionized water evenly, sterilize in an autoclave at 110°C for 35 minutes after mixing evenly, and cool to room temperature after sterilization to obtain a mixed solution;

[0048] (2) Insert lactic acid bacteria into the mixed solution for fermentation, wherein the lactic acid bacteria are Lactobacillus plantarum and Lactobacillus bulgaricus, and the number of viable bacteria in the lactic acid bacteria liquid is 10 9 CFU / mL, based on the volume of deionized water (120mL) in the initial fermentation system, the inoculation amount of lactic acid bacteria is 0.2vol%, and the ratio of viable bacteria of the Lactobacillus plantarum and Lactobacillus bulgaricus is 1:4, at 30°C Cultivate in a constant temperature oscillator for 24 hours, and the shaking table speed is 150r / min; after the fermentation is completed...

Embodiment 3

[0051] The preparation method of the peony fermentation stoste described in the present embodiment, comprises the steps:

[0052] (1) Mix 6g of powdered peony and 600mL of deionized water evenly, sterilize in an autoclave at 125°C for 15 minutes after mixing evenly, and cool to room temperature after sterilization to obtain a mixed solution;

[0053] (2) Insert lactic acid bacteria into the mixed solution for fermentation, wherein the lactic acid bacteria are Lactobacillus plantarum and Lactobacillus bulgaricus, and the number of viable bacteria in the lactic acid bacteria liquid is 10 11 CFU / mL, based on the volume of deionized water (600mL) in the initial fermentation system, the inoculum size of lactic acid bacteria is 1vol%, the ratio of viable bacteria of the Lactobacillus plantarum and Lactobacillus bulgaricus is 4:1, at a constant temperature of 45°C Cultivate in a shaker for 12 hours, and the rotating speed of the shaking table is 300r / min; after the fermentation, the ...

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Abstract

The invention discloses a peony fermentation stock solution as well as a preparation method and application thereof. The method comprises the following steps: (1) grinding dry peony plants into powder, and sieving to obtain powdery peony; (2) mixing the powdery peony with water, sterilizing, and cooling to room temperature to obtain a mixed solution; (3) inoculating lactic acid bacteria into the mixed liquid for fermentation culture, sterilizing after fermentation is finished, and centrifuging to take supernate to obtain peony initial fermentation liquid; (4) adding a decolorizing agent into the peony initial fermentation liquid, stirring and decolorizing, then carrying out suction filtration and sterilization, and then adding a preservative to obtain a peony fermentation stock solution; the zymophyte is lactobacillus plantarum and lactobacillus bulgaricus. According to the preparation method, the peony is fermented by adopting the lactobacillus plantarum and the lactobacillus bulgaricus, so that the loss of active ingredients in the peony is reduced, and the prepared peony fermentation liquor has relatively good effects of resisting oxidation, whitening and nourishing skin and has a very good application prospect in the field of cosmetics.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a fermented stock solution of peony and its preparation method and application. Background technique [0002] On the basis of a large number of experimental evidences, the theory of free radicals is the most valued among the modern aging theories. The theory points out that the body cells produce more active oxygen free radicals in the normal metabolic process. Free radicals are in a dynamic balance of continuous generation and removal in the human body. When the human body is affected by exogenous factors and endogenous factors, too much free radicals or too little removal may cause damage to tissue cells , And then cause the aging of the body and other diseases. Peony has a long history in China. In addition to its important ornamental function, its cultural value, medicinal value, edible value, economic benefit, and ecological benefit are remarkable. With the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/08A61Q19/02A61Q19/00
CPCA61K8/9789A61Q19/08A61Q19/02A61Q19/00A61K2800/85
Inventor 韩婕珺龚天贵王海涛
Owner 美出莱(杭州)化妆品有限责任公司