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Method for accurately, quantitatively and rapidly detecting ovarian cancer in early stage

An ovarian cancer, rapid technology, applied in the field of genetic engineering antibodies and food biology, can solve the problems of expensive equipment, affecting the progress of the experiment, and dropping test tubes, and achieve the effect of promoting electron transfer, improving convenience, and improving accuracy

Inactive Publication Date: 2022-03-22
HANGZHOU FIRST PEOPLES HOSPITAL
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

DNA methylation is closely related to growth and development, aging, gene expression regulation, especially the occurrence and development of various tumors including ovarian cancer; currently, many overall methylation level detection methods are cumbersome and expensive. Each has certain limitations; during the detection process, the sample needs to be put into the test tube, and then uniformly entered into the detection device for detection, but because the existing microplate plate is easy to let the test tube drop, affecting the progress of the experiment

Method used

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  • Method for accurately, quantitatively and rapidly detecting ovarian cancer in early stage
  • Method for accurately, quantitatively and rapidly detecting ovarian cancer in early stage
  • Method for accurately, quantitatively and rapidly detecting ovarian cancer in early stage

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Embodiment Construction

[0033] like Figure 1-14 As shown, a method for early accurate and quantitative rapid detection of ovarian cancer comprises the following steps:

[0034] a. Using nanomaterial labeling technology to bind specific recognition molecules to the surface of nanoparticles to construct a biofunctional nanoparticle sensing interface;

[0035] b. Based on the adsorption of nanoparticles, DNA fragments are immobilized on the surface, and after treatment with methyltransferase, 5'methylcytosine is detected by enzyme-labeled anti-5'methylcytosine antibody;

[0036] c. Put the sample into the ELISA plate, first put the sample into the test tube, and then place the test tube on the moving plate 1; press the button 10, drive the clamping device 2 to start, and fix the periphery of the test tube; the clamp The tightening device 2 includes a fixed plate 20, a semicircular groove 21, a clamping mechanism 4, a first rack 23, and a first gear 24; 20 can be relatively movable and be located on t...

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Abstract

The invention discloses a method for accurately, quantitatively and rapidly detecting ovarian cancer in an early stage. The method comprises the following steps: a, constructing a biologically functionalized nanoparticle sensing interface; b, detecting 5 '-methylcytosine; c, putting the sample into an elisa plate, and carrying out specific and rapid qualitative and quantitative detection on the overall genome level in the sample through the change of electrochemical signals such as impedance, potential and current caused by a labeled enzyme catalysis substrate on the surface of the sensor; according to the method, the property of a nano material can be adopted to promote electron transfer on the surface of the sensor, so that a signal amplification effect is achieved, and the nano material is used as a sensing interface to establish a bio-functionalized screen-printed electrode; the detection convenience is improved, and convenience is further provided for rapid detection of the ovarian cancer.

Description

technical field [0001] The invention belongs to the field of genetic engineering antibody and food biotechnology, and in particular relates to a method for early, accurate, quantitative and rapid detection of ovarian cancer. Background technique [0002] Ovarian cancer is a common malignant tumor of the female reproductive system, its incidence rate is the third among female reproductive system tumors, and its mortality rate ranks first among gynecological malignancies. There are about 190,000 new cases of ovarian cancer worldwide each year, and about 114,000 deaths each year; the onset of ovarian cancer is hidden, and 70% of ovarian cancer patients are diagnosed at an advanced stage[2]. The 5-year survival rate of patients with early-stage ovarian cancer can reach 90% due to the development of debulking surgery and effective chemotherapy regimens. Due to the limitations of current diagnosis and treatment techniques and the non-specific clinical symptoms of patients, the di...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327G01N33/53G01N33/574G01N33/58
CPCG01N27/26G01N27/3271G01N27/3278G01N33/57449G01N33/57484G01N33/5308G01N33/581G01N33/5302
Inventor 逯岭松刘蓓马宵周建平
Owner HANGZHOU FIRST PEOPLES HOSPITAL