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Intelligent instant quantitative detection device for mycotoxin quantum dot test strip

A mycotoxin and test strip technology, which is applied in the field of intelligent real-time quantitative detection devices for mycotoxin quantum dot test strips, can solve the problems of unfavorable mycotoxin supervision and risk assessment, long detection process, changes in mycotoxin content, etc., and achieve improved Portability and practicality, avoidance of use, effect of solving technical problems

Active Publication Date: 2022-03-25
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because fungi are easily affected by climate, soil and storage conditions, etc., and the sample delivery, pretreatment and detection process in the laboratory chromatography mass spectrometry detection is relatively long, resulting in changes in the content of mycotoxins, which is not conducive to the supervision and risk assessment of mycotoxins

Method used

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  • Intelligent instant quantitative detection device for mycotoxin quantum dot test strip
  • Intelligent instant quantitative detection device for mycotoxin quantum dot test strip
  • Intelligent instant quantitative detection device for mycotoxin quantum dot test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1 (agricultural product food)

[0075] Detection of aflatoxins and zearalenone in corn:

[0076] Weigh 60 g of corn kernels, add 60 mL of acetonitrile / water solution (80:20, v:v), homogenize at 18000 rpm for 2 minutes, and filter with glass fiber filter paper. The filtrate was collected, and 1 mL of the filtrate was added to 3 mL of sample diluent (PBS buffer containing 1% BSA, 1% sucrose, 2% tween-20, 0.5% PVPK-30, pH 7.4, 0.01M). Take 200 μL of the mixed solution and 1 μL of the quantum dot fluorescent probe in the enzyme-labeled microwell, insert the mycotoxin quantum dot test strip, and incubate at 37°C for 6 minutes. Remove the test strip. Insert it into the test strip card slot 17 of the housing 1, take pictures with the mobile phone, collect and convert the picture data, and obtain the contents of aflatoxin and zearalenone in corn.

Embodiment 2

[0077] Embodiment 2 (life and health)

[0078] Detection of aflatoxin and zearalenone in urine:

[0079] Take 10 mL of urine and filter it with quantitative filter paper, take 1 mL of filtered urine, add 1 mL of sample diluent (PBS buffer containing 2% BSA, 2% sucrose, 4% tween-20, 1% PVPK-30, pH 7.4, 0.02M). Take 200 μL of the mixed solution and 1 μL of the quantum dot fluorescent probe in the enzyme-labeled microwell, insert the mycotoxin quantum dot test strip, and incubate at 37°C for 6 minutes. Remove the test strip. Insert it into the test strip slot 17 of the housing 1, take pictures with the mobile phone, collect and convert the picture data, and obtain the contents of aflatoxin and zearalenone in the urine.

[0080] technical effect

[0081] 1. The first light source 2 and the second light source 3 excite the test strip placed in the housing 1 and sprayed with the mycotoxin antigen coupled with the hyperbranched DNA tetrahedral pendant nanomaterial to obtain emi...

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Abstract

The invention discloses a mycotoxin quantum dot test strip intelligent instant quantitative detection device. The first light source and the second light source are used for exciting the test strip which is placed in the shell and sprayed with the mycotoxin antigen to obtain emitted light, and the light filtering component is used for filtering out impurity light. The clamping mechanism is used for clamping and fixing the shooting part, then the shooting part is used for capturing a fungaltoxin quantum dot test strip picture, and the processor is used for analyzing and calculating picture information to obtain a fungaltoxin content result, so that the detection time is shortened, and the portability and practicability of fungaltoxin detection are improved; quantitative instant detection of single or multi-toxin mixed pollution is realized, and the technical problem of fluorescence quantification is solved.

Description

technical field [0001] The invention relates to the technical field of mycotoxin detection, in particular to an intelligent instant quantitative detection device for mycotoxin quantum dot test strips. Background technique [0002] Food safety is a major issue related to my country's national economy and people's livelihood. my country's food is susceptible to mixed contamination of various mycotoxins, which seriously threatens national life and health and economic and trade security. Mycotoxins are secondary metabolites produced by fungi and are one of the most dangerous food contaminants naturally occurring. About 4.5 billion people worldwide are exposed to the risk of mycotoxins for a long time. More than 300 mycotoxins have been isolated and identified. Among them, aflatoxin B1 (AFB1) is designated as a class I carcinogen by the International Agency for Research on Cancer (IARC), which can induce liver cancer and has teratogenic and mutagenic effects. OTA has nephrotoxic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N33/558G01N33/543
CPCG01N21/8483G01N33/558G01N33/54306G01N21/6456G01N33/54388G01N2333/37G01N33/588G01N33/56961G01N21/78G01N2021/7759G01N2021/7786
Inventor 胡小风张兆威李培武姜俊张文
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI