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Application of Liyun type fructan

A technology for fructan and germs, which is applied in the application field of Liyun-type fructan, and achieves the effects of not easy drug resistance and broad control spectrum.

Active Publication Date: 2022-04-05
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the excessive use of pesticides has also brought severe challenges to the quality and safety of agricultural products and the ecological environment.

Method used

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  • Application of Liyun type fructan
  • Application of Liyun type fructan
  • Application of Liyun type fructan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of embodiment 1 Liyun type fructan

[0022] The method (Burak AdnanErkorkmaz et al, 2018DOI: 10.1007 / s00449-018-1952-x) of reference Burak Adnan Erkorkmaz for the preparation of Levan type fructan, is about to inoculate Halomonas Smyrna AAD6 (T) in fermentation medium, The fermentation medium contains the following components: 137.2g / L NaCl, 50g / L sucrose, 7g / L K 2 HPO 4 , 2g / L KH 2 PO 4 , 0.1g / L MgSO 4 ·7H 2 O, 1g / L (NH 4 ) 2 SO 4 , 0.5g / L casein peptone, 50mM H 3 BO 3 , 0.8mg / L Thiamine, trace elements (0.36mg / L MnCl 2 4H 2 O, 0.44mg / L ZnSO 4 ·7H 2 O, 2.3mg / LFeSO 4 ·7H 2 O, 0.05mg / L CuSO 4 ·5H 2 0), adjust the pH to 7.0 with 5M NaOH and HCl solution, and make up to 1 L with water. Among them, the thiamine and trace element solutions were sterilized by filtration, and the sucrose solution was autoclaved at 121° C. for 3 minutes to avoid sugar caramelization. NaCl, mineral solutions, NaOH, HCl and boron source solutions were autoclaved...

Embodiment 2

[0023] The processing of embodiment 2 Liyun type fructan aqueous agent to plant material

[0024] A kind of Levant type fructan aqueous preparation, is made up of the component of following weight and volume percentage content (g / 100mL): Levant type fructan 0.5%, water supplements to 100% (for spraying, need to add 0.0001 % of Tween-20 as surfactant).

[0025] In this experiment, Yanmai No. 3 was used as the plant material, and the treatment included three methods: soaking, spraying, and soaking+spraying. The specific steps were: select the seeds with full grains, soak them in 1% sodium hypochlorite solution for 10 minutes, rinse them with sterile water, Blot the water on the surface of the seeds with sterile filter paper, soak them in water or Liyun-type fructan water for 24 hours. Refer to the national standard GB / T 7416-2008, put the seeds in a petri dish lined with double-layer moist filter paper, seal it and place it in a dark incubator at 20°C and 80% humidity for 3 day...

Embodiment 3

[0026] Example 3 Liyun type fructan water treatment to the control effect of head blight

[0027] Inoculate Fusarium graminearum F0609 onto a PDA plate, activate in the dark at 25°C for 3 days, pick a small amount of mycelium and inoculate it into mung bean medium, culture at 180r / min with shaking at 25°C for 3 days, and pass through 8 layers of gauze to collect spores. Adjust the concentration of spore fluid to 1×10 6 / ml, vortexed to mix. The inoculation method adopts the piercing method, pierces the middle part of the leaf described in Example 2 with a needle with a diameter of 1 mm, draws 5 μl of spore liquid and drips it on the perforation, covers the lid of the culture dish, seals it and places it in an incubator at 25° C. / dark 12h alternate cultivation, after 2d, carry out lesion area analysis and disease severity classification (results see figure 1 ).

[0028] According to the size of the lesion area, the severity of the disease is divided into three categories: l...

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Abstract

The invention provides an application of a Liyun type fructan. The fructosan provided by the invention is a Liyun type fructosan synthesized from Halomonas sp. AAD6 (T), and the fructosan is non-toxic and harmless, does not have antibacterial activity, but can activate the immunity of plants so as to improve the capability of resisting gibberellic disease and banded sclerotial blight. Meanwhile, the Liyun type fructan can be dissolved in water, no organic solvent needs to be added when the Liyun type fructan is used, and the requirement of green pesticides is met.

Description

technical field [0001] The invention provides an application of Liyun-type fructan, which belongs to the field of green pesticides. Background technique [0002] Wheat (Triticum aestivum L.) is the main grain crop in my country and occupies an important position in China. Its planting area accounts for 22%-30% of the national arable land area, mainly concentrated in the lower reaches of the Yellow River Basin. As the sown area of ​​wheat decreases year by year, it is particularly important to ensure wheat production. Fusarium head blight (Fusarium head blight), also known as ear rot, is one of the important diseases that cause wheat yield reduction. It is caused by a variety of Fusarium fungi and can occur throughout the growth period, resulting in seedling rot, stem rot, and ear rot. In popular years, head blight makes wheat susceptible to 50-100% and reduces yield by 10-20%. The toxin it produces also indirectly threatens the health of humans and animals. [0003] Rice (...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P3/00A01P21/00
Inventor 梁明祥水晨连丹
Owner NANJING AGRICULTURAL UNIVERSITY
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