CrRNA primer pair for PPV detection, CRISPRCas12a system and application method
An application method and primer pair technology, which is applied in the field of crRNA primer pair and CRISPRCas12a system, can solve the problems of invisible infection and infected pigs without obvious clinical symptoms, and achieve the effects of improving detection efficiency, reducing detection threshold, and saving detection time
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[0060] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0061] The present invention provides a technical solution: a crRNA primer pair for PPV detection, a CRISPRCas12a system and an application method, comprising the following steps:
[0062] S1. Treatment of tissue samples: Weigh about 1 g of each tissue sample, add 1 mL of PBS, put it into a grinding bowl and grind it into a homogenate, transfer it to a sterile centrifuge tube, and centrifuge at 12000 rpm / min for 3 min in a centrifuge;
[0063] S2. Extraction o...
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