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Monascus B with glycosidase inhibition activity and immunomodulatory activity and preparation method of monascus B

An immunomodulatory activity and glycosidase technology, which is applied in the fields of extraction, structure identification, activity determination and purification of monascus B, can solve the problems of few types of hypoglycemic drugs and small choices, and achieve huge application potential and economical Value, easy to control, high success rate effect

Pending Publication Date: 2022-04-08
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]In recent years, due to the improvement of living standards and the reduction of exercise, the number of diabetic patients has increased year by year. At present, the number of diabetic patients in the world has reached 440 million, and the proportion is 5% every year increase, but currently there are few types of hypoglycemic drugs, and there is little room for choice, so it is urgent to develop new high-efficiency hypoglycemic drugs

Method used

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  • Monascus B with glycosidase inhibition activity and immunomodulatory activity and preparation method of monascus B
  • Monascus B with glycosidase inhibition activity and immunomodulatory activity and preparation method of monascus B
  • Monascus B with glycosidase inhibition activity and immunomodulatory activity and preparation method of monascus B

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A kind of operation steps of Monascus B with glycosidase inhibitory activity and immunomodulatory activity are as follows:

[0049] (1) Preparation of solid culture

[0050] The bacterial classification of Monascus purpureus is subjected to primary strain cultivation, secondary seed cultivation and tertiary liquid expansion cultivation to obtain a liquid culture;

[0051] (1.1) Primary strain culture

[0052] will be 10 7 spores / mL Monascus purpureus (Monascus purpureus) spore suspension was inoculated on the potato agar (PDA) slant medium, each slant was inoculated with 10 μL of the spore suspension, and cultivated for 7 days at a temperature of 32 ° C and a rotation speed of 220 r / min to obtain First-class bacteria.

[0053] PDA medium is potato dextrose medium, the formula is: 200g peeled potato, 20g glucose and 15g agar, add water to 1000mL.

[0054] (1.2) Secondary seed cultivation

[0055] Make the first-grade bacteria with sterile water to a concentration of...

Embodiment 2

[0074] A kind of operation steps of Monascus B with glycosidase inhibitory activity and immunomodulatory activity are as follows:

[0075] (1) Preparation of solid culture

[0076] will be 10 6 The liquid strain of spore / mL Anka Monascus anka (Monascus anka) obtains the liquid culture through primary strain cultivation, secondary seed cultivation and tertiary liquid expansion cultivation;

[0077] (1.1) Primary strain culture

[0078] The spore suspension of Monascus anka was inoculated on the potato agar (PDA) slant medium, and each slant was inoculated with 30 μL of the spore suspension, and cultivated for 9 days at a temperature of 35 ° C and a rotation speed of 250 r / min. Get first-class strains.

[0079] PDA medium is potato dextrose medium, the formula is: 200g peeled potato, 20g glucose and 15g agar, add water to 1000mL.

[0080] (1.2) Secondary seed cultivation

[0081] Make the first-grade bacteria with sterile water to a concentration of 10 7 spores / mL of spore...

Embodiment 3

[0100] A kind of operation steps of Monascus B with glycosidase inhibitory activity and immunomodulatory activity are as follows:

[0101] (1) Preparation of solid culture

[0102] will be 10 7 The bacterial classification of spore / mL Monascus ruber (Monascus ruber) obtains liquid culture through primary strain cultivation, secondary seed cultivation and tertiary liquid expansion cultivation;

[0103] (1.1) Primary strain culture

[0104] Inoculate the spore suspension of Monascus ruberus on the potato agar (PDA) slant medium, inoculate 20 μL of the spore suspension on each slant, and cultivate it for 8 days at a temperature of 33.5 ° C and a rotation speed of 235 r / min to obtain a first-class strain .

[0105] PDA medium is potato dextrose medium, the formula is: 200g peeled potato, 20g glucose and 15g agar, add water to 1000mL.

[0106] (1.2) Secondary seed cultivation

[0107] Make the first-grade bacteria with sterile water to a concentration of 10 6 225 μL per bottl...

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Abstract

The invention relates to monascus B with glycosidase inhibition activity and immunomodulatory activity and a preparation method thereof, and belongs to the technical field of extraction and preparation of biological products. The monascus B is red powder, and the molecular formula of the monascus B is C23H30O6; the compound has the advantages that the compound has the activity of inhibiting glycosidase, the median inhibitory concentration (IC50) on alpha-glycosidase is 174 mu g / mL, and the transcriptional level of IL-1beta, TNF-alpha and TGF-beta of in-vitro cultured mouse macrophages RAW264.7 is obviously reduced: the change of the IL-1beta (0.01 + / -0.01), the change of the TNF-alpha (0.02 + / -0.01) and the change of the TGF-beta (0.03 + / -0.01) relative to a DMSO (dimethylsulfoxide) control group are obvious (P is less than 0.05). The monascus purpureus B is obtained by culturing monascus purpureus and then extracting and separating the monascus purpureus. The monascus used in the invention is an edible fungus and is safe and reliable, and the preparation method adopts microbial fermentation production, is environment-friendly, is not influenced by natural environment and resources, and is easy to realize industrial, automatic and continuous production.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of biological products, and in particular relates to the extraction, purification, structure identification and activity determination of monascus kaolin B, which has glycosidase inhibitory activity. Background technique [0002] Monascus sp. is a small filamentous saprophytic fungus, belonging to Eumycophyta, Ascomycotina, Plectomycetes, Eurotiales, Monascus Family (Monascaceae), Monascus (Monascus), including species are Monascus purpureus, Monascus anka and Monascus ruber. Monascus can produce glucoamylase, esterase, protease and other enzymes. Under the synergy of these enzymes, it can produce monosaccharides, amino acids, nucleotides and other taste substances and volatile acids, alcohols and Aroma substances such as esters contribute significantly to the flavor of fermented foods. [0003] In recent years, due to the improvement of living standards and the reduction of e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A61P37/02A61P29/00A61P3/10C12P17/18C12R1/645
Inventor 陆瑞利黄永芳胡丰林
Owner ANHUI AGRICULTURAL UNIVERSITY
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