Application of CircHOMER1 and colorectal cancer treatment preparation

A colorectal cancer and preparation technology, applied in the field of tumor molecular biology, can solve the problems of circRNA function without research reports, etc., achieve the results of authentic and reliable results, rigorous test process, and improve the effect of treatment efficacy

Pending Publication Date: 2022-04-12
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Studies have shown that non-coding RNA (lncRNA and miRNA) can play a role in the process of photodynamic therapy of tumors, and the function of circRNA in the process of photodynamic therapy of tumors has not been reported so far.

Method used

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  • Application of CircHOMER1 and colorectal cancer treatment preparation
  • Application of CircHOMER1 and colorectal cancer treatment preparation
  • Application of CircHOMER1 and colorectal cancer treatment preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Example 1: RNA-seq experiment

[0053] RNA-Seq sequencing was performed on the cells before and after PDT treatment, and the differentially expressed circRNAs after PDT treatment were screened. This part of the experiment was completed by Guangzhou Ruibo Biotechnology Co., Ltd. The specific steps are as follows: After RNA extraction and quality inspection, the rRNA was removed by the probe, and the linear RNA was removed by RNaseR to construct the Illumina sequencing library. Sequencing was then performed, the sequencing mode was PE150, and the sequencing data volume was 12G / 15G. For the sequencing data, first perform Raw Data filtering, de-joining sequences and low-quality reads to obtain high-quality data (Clean Data). Then, by comparing with the ribosome database, the ribosomal RNA sequence is removed to obtain valid reads. Valid reads are compared with the reference genome (mapping), and the comparison results are used for the identification of circular RNAs in th...

Embodiment 2

[0058] Example 2: qPCR detection of expression of circular RNA circHOMER1 in colorectal cancer tissue

[0059] 40 pairs of fresh tissues were collected for qPCR to detect the expression of circular RNA circHOMER1 in colorectal cancer and adjacent tissues. The results are as follows: image 3 shown. Circular RNA circHOMER1 was highly expressed in paracancerous tissues and low in colorectal cancer tissues.

Embodiment 3

[0060] Example 3: qPCR detection of the expression of circular RNA circHOMER1 after photodynamic treatment

[0061] After colorectal cancer cells RKO and CX-1 were incubated with complete medium (containing 0.1 μM photosensitizer) for 4 hours, 10 cm 2 / J laser treatment. After continuing to culture for 6 hours, the cells were collected and Trizol was added to lyse the cells and the expression of circular RNA circHOMER1 was detected.

[0062] The expression levels of circular RNA circHOMER1 in RKO, RKO-PDT and CX-1, CX-1-PDT group cells were detected by qPCR. Such as Figure 4 As shown, the expression level of circular RNA circHOMER1 was significantly upregulated in RKO and CX-1 cells after photodynamic treatment.

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Abstract

The invention discloses an application of circHOMER1 and a colorectal cancer treatment preparation. Researches prove that circHOMER1 is low in expression in colorectal cancer tissues, and the sensitivity of photodynamic treatment on colorectal cancer tumor cells can be improved by overexpressing circHOMER1. Therefore, the circHOMER1 is used as a reagent for improving the photodynamic therapy, has profound clinical significance and has the application prospect of improving the photodynamic therapy of the colorectal cancer.

Description

technical field [0001] The invention belongs to the technical field of tumor molecular biology, and specifically relates to the application of a circular RNA circHOMER1 in the preparation of a reagent for improving the photodynamic therapy of colorectal cancer and the corresponding preparation for treating colorectal cancer. Background technique [0002] Colorectal cancer is a common malignant tumor of the digestive system. In China, the morbidity and mortality of colorectal cancer rank 4th and 5th among all malignant tumors, and the new cases of colorectal cancer in my country are related to colorectal cancer. The death toll ranks first in the world. The disease poses a serious threat to health and imposes a heavy economic burden on society and individuals. Common tumor treatment methods include surgery, radiotherapy, and chemotherapy. Conventional treatment has severe side effects, and often fails to achieve good results for patients who are resistant to radiotherapy and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7088A61P1/00A61P35/00C12N15/113C12N15/85
Inventor 黄河徐容林良武熊力
Owner CENT SOUTH UNIV
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