ABHD2 gene expression inhibitor and application and medicine thereof

A technology of gene expression and inhibitors, applied in the field of ABHD2 gene expression inhibitors, can solve the problems that have yet to be revealed, and achieve the effect of enriching molecular mechanisms, promoting the development process, and promoting the apoptosis of liver cancer cells

Active Publication Date: 2022-04-12
XINXIANG MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the potential role of TDP-43 in the development of liver cancer and related molecular mechanisms still remain to be revealed.

Method used

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  • ABHD2 gene expression inhibitor and application and medicine thereof
  • ABHD2 gene expression inhibitor and application and medicine thereof
  • ABHD2 gene expression inhibitor and application and medicine thereof

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Embodiment 1

[0028] 1. Materials and methods

[0029] 1.1 Cell culture

[0030] Human liver cancer cells SMMC-7721, MHCC97H, Huh-7 and HEK293T cell lines were purchased from Suzhou Beina Chuanglian Biotechnology Co., Ltd. Cultured in a 37°C incubator under carbon dioxide concentration.

[0031] 1.2 Plasmid construction

[0032] Using the mRNA extracted from SMMC-7721 cells as a template, the coding region sequence (CDS) of TDP-43 was amplified by RT-PCR, and the enzyme cutting sites XhoI and BamHI were introduced during the amplification, and then the sequence and the vector pEGFP-C1 were respectively Carry out double enzyme digestion, use T4 DNA ligase to connect the PCR products of the above enzyme digestion with the pEGFP-C1 vector, and finally construct the recombinant plasmid pEGFP-TDP-43 for the exogenous overexpression of TDP-43. Using the same method, the ABHD2 CDS was cloned into the pCMV3 vector to construct pCMV3-ABHD2 for ABHD2 overexpression. The 3'UTR of ABHD2 was amplifi...

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Abstract

The invention relates to the field of biological medicine, in particular to an ABHD2 gene expression inhibitor, application thereof and a medicine. The inhibitor is one or two of 1) and 2): 1) a product which is prepared or screened by taking the ABHD2 gene as a target and has an inhibition effect on the ABHD2 gene; and 2) a product which is obtained by taking the upstream regulatory gene of the ABHD2 gene as a target or screening and has an inhibition effect on the upstream regulatory gene. The result of the invention shows that the TDP-43 can be combined with the ABHD2 mRNA 3 'UTR and enhance the stability of the ABHD2 mRNA, so that the expression of the ABHD2 is up-regulated. According to the application, the TDP-43 affects the lipid metabolism process through the ABHD2, then liver cancer cell apoptosis is inhibited, the development process of liver cancer is finally promoted, liver cancer cell apoptosis can be promoted and liver cancer cell proliferation can be inhibited by down-regulating expression of the ABHD2 or the TDP-43, and the result has important theoretical guidance significance and clinical application value for precise treatment of liver cancer.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an ABHD2 gene expression inhibitor, its application and medicine. Background technique [0002] For many years, primary hepatocellular carcinoma (hereafter referred to as liver cancer) has been endangering human life and health, and is one of the most common malignant tumors in the world. The complex pathogenesis of HCC involves multiple processes such as chronic inflammation, fibrosis, and oncogene dysregulation. There are various treatments for liver cancer, depending on the stage of the cancer and the condition of the patient. Liver cancer can be treated mainly by means of liver transplantation, hepatectomy, radiofrequency ablation, transcatheter arterial chemoembolization, and targeted therapy. However, so far, the five-year survival rate of liver cancer patients is still low, with only one-third of patients living an average of more than 60 months after surgical resection, trans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/7088A61P35/00
Inventor 刘博雯曹金玲王相允申志发陈露露周佳王伊蕾
Owner XINXIANG MEDICAL UNIV
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