Method for improving poultry production efficiency by rapidly evaluating liver brittleness
A production efficiency and poultry technology, applied in the field of poultry breeding, can solve the problems of expensive B-type ultrasonic detector, poultry is not easy to accept security quietly, and liver fragility cannot be judged, etc., achieving low price, high coefficient of determination, and accuracy high effect
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Embodiment 1
[0022] 1. Establish a regression equation for estimating liver fragility in vivo
[0023] (1) Select Rugao yellow chickens as the modeling group, at 4 time points of 18 weeks, 22 weeks, 28 weeks, and 36 weeks, randomly select 10 chickens at each time point, and collect blood through wing veins, Perform anticoagulant treatment, collect plasma directly for testing or freeze at -20°C for later use;
[0024] (2) Measure the total cholesterol in the blood of each sample (as shown in Table 1), then use the curve evaluation regression method to carry out curve fitting to the above-mentioned blood biochemical index total cholesterol and liver fragility, and establish a regression equation y=-0.007x 3 +0.140x 2 –0.933x+2.744 (where R 2 =0.816, x is the content of total cholesterol in individual sample blood, the unit is mmol / L), and the t test shows that the P values of the regression coefficients -0.007, 0.140, and 0.933 are all less than 0.001, which is significant; according to ...
Embodiment 2
[0036] 1. Selection of blood biochemical indicators when establishing a regression equation for estimating liver fragility in vivo
[0037] (1) Select Rugao yellow chickens as the modeling group, at 4 time points of 18 weeks, 22 weeks, 28 weeks, and 36 weeks, randomly select 10 chickens at each time point, and collect blood through wing veins, Perform anticoagulant treatment, collect plasma directly for testing or freeze at -20°C for later use;
[0038] (2) Determination of ALT (U / L), AST (U / L), total cholesterol (mmol / L), and triglyceride (mmol / L) in the blood of each sample, respectively, compared with those measured by the muscle tenderness instrument Correlation analysis, linear regression analysis and curve estimation were performed on liver shear force.
[0039] (3) The correlation analysis between ALT and the liver shear force measured by the muscle tenderness instrument did not reach significance (the correlation coefficient was -0.167, P=0.297), and was eliminated. ...
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