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Exogenous radish fragment specific marker as well as preparation method and application thereof

A technology of specific markers and radishes, applied in botany equipment and methods, biochemical equipment and methods, applications, etc., can solve problems such as unstable inheritance, achieve genetic stability, overcome the serious low heritability of restored genes, and eliminate mechanical mixed effect

Active Publication Date: 2022-04-15
湖南省作物研究所
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

For example, Li Xufeng reported that the restorer gene was introduced from the radish var. indigo to rapeseed, but the restorative genes of the obtained materials were all on the episomes, but could not be stably inherited; After crossing with a sterile line, the F 1 In order to genetically improve R2000, the recovered individual plants with changed markers were screened for radiation mutagenesis, but the recovered sources and molecular markers used were all from foreign research.
There is no self-developed molecular marker in our country that can be used for ogura-CMS restorer line breeding

Method used

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  • Exogenous radish fragment specific marker as well as preparation method and application thereof
  • Exogenous radish fragment specific marker as well as preparation method and application thereof
  • Exogenous radish fragment specific marker as well as preparation method and application thereof

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Embodiment 1

[0054] The breeding method of the cytoplasmic sterile restorer line CLR095 of Brassica napus radish in the embodiment of the present invention is shown in the attached figure 1 As shown, it specifically includes the following steps.

[0055] 1. Development of specific molecular markers and linkage markers of radish cytoplasmic male sterility restorer gene

[0056] 1.1 Implementation materials

[0057] Brassica oleracea radish sterility recovery material CLR650 (it is radish male sterile cytoplasm, leaf edge is zigzag, leaf tip is sharper, leaf color is dark green, siliques are thick and short, see literature: Chen Weijiang et al., Brassica oleracea radish Creation of cytoplasmic male sterility restoration material, Agricultural Sciences of China, 2012, 45(8): 1465-1474), the material is a hybrid of Raphanobrassica (AACCRR, 2n=56) and Brassica napus through grafting technology Breeding, the number of chromosomes is between 38-40. Its cytoplasm is radish male sterile cytoplas...

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Abstract

The invention relates to a brassica napus exogenous radish fragment specific marker and a preparation method and application of the exogenous radish fragment specific marker, and specific molecular markers CLR9-1-CLR9-20 of the exogenous radish fragment are developed by comparing whole genome re-sequencing data of a brassica napus radish cytoplasm sterility restoring material CLR650 with radish genome sketch information. A BC4 segregation population is obtained through a target single plant of a cytoplasm sterility restoration material CLR650 backcross population; carrying out multi-generation mixed selection on fertile plants in the BC4 population by adopting a mixing method until a mixed segregation population with the fertile plant proportion exceeding 75% is obtained; finally, homozygous stabilization is performed through multi-generation selfing, and the cabbage type rape radish cytoplasm sterility restoring line is obtained. The method overcomes the defects that the heritability of the recovered gene is seriously low and the character is difficult to homozygous and stabilize in the existing method.

Description

[0001] (This application is a divisional application of "a specific marker for exogenous radish fragments and its preparation method and application", the filing date of the original application is August 24, 2017, the application number is 201710735768.7, and the name of the invention is "a Specific markers for exogenous radish fragments and their preparation methods and applications.") technical field [0002] The invention belongs to the technical field of rapeseed breeding and the field of molecular biology, and in particular relates to a specific marker for exogenous radish segments and a preparation method for specific markers for exogenous radish segments, and also relates to the application of specific markers for exogenous radish segments in the breeding of Brassica napus . Background technique [0003] Rapeseed has obvious heterosis, and is the oil crop with the most potential to cope with the shortage of edible vegetable oil and develop renewable bioenergy at home...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6895C12Q1/6806A01H1/04A01H1/02
Inventor 王同华郭一鸣涂金星陈卫江李莓范连益曲亮惠荣奎
Owner 湖南省作物研究所
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