Method for simultaneously detecting four mycotoxins based on multi-channel label-free electrochemical aptamer sensor

A technology of aptamer sensor and mycotoxin, which is applied in the field of electrochemical analysis, achieves the effects of low detection cost, simple preparation process and high specificity

Pending Publication Date: 2022-04-15
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still a gap in the method for the simultaneous detection of multiple mycotoxins in crops based on the EIS method

Method used

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  • Method for simultaneously detecting four mycotoxins based on multi-channel label-free electrochemical aptamer sensor
  • Method for simultaneously detecting four mycotoxins based on multi-channel label-free electrochemical aptamer sensor

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Embodiment 1

[0045] (1) Preparation and pretreatment of multi-channel ITO electrodes

[0046] Such as figure 1 As shown, cut the ITO conductive glass into a rectangle of 35×25mm, and then use laser etching to leave the required four working areas a, b, c, d (diameter = 6mm) and their respective conductive channels. Then the multi-channel ITO electrode was sonicated in acetone, ethanol and water for 10 min, respectively. Then, dry the multi-channel ITO electrode under an infrared lamp, and cover the surface of the ITO electrode with a brown adhesive tape with four round holes (25×25mm, the diameter of the round hole is 6mm, and the position coincides with the working area of ​​the electrode).

[0047] (2) Four working areas to modify gold nanoparticles

[0048] In the three-electrode system, the reference electrode is a saturated calomel electrode, the counter electrode is a platinum wire electrode, and the working electrode is a multi-channel ITO electrode. in 2mM HAuCl 4 Electrodeposi...

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Abstract

The invention belongs to the field of electrochemical analysis, and discloses a method for simultaneously detecting four mycotoxins based on a multi-channel label-free electrochemical aptamer sensor. Comprising the following steps: preparing and preprocessing a multi-channel ITO chip; the four working areas are modified with gold nanoparticles (ITO-AuNPs); the four nucleic acid aptamers are modified on the surfaces of the gold nanoparticles (ITO-AuNPs-Apt); sealing redundant active sites in the working area; the electrochemical impedance method is used for simultaneously detecting FB1, AFB1, OTA and ZEN. According to the present invention, based on the specific recognition effects of the nucleic acid aptamer and the target objects such as FB1, AFB1, ZEN and OTA, the multi-channel working electrode is adopted, the electrochemical workstation is adopted to achieve the signal output, the simultaneous detection of the four mycotoxins is achieved, the characteristics of high specificity, strong stability and the like are provided, and the pollution condition of crops can be well indicated. Meanwhile, the method has the characteristics of simple preparation process, short time consumption in the detection process, small reagent dosage, low detection cost and the like.

Description

technical field [0001] The invention belongs to the field of electrochemical analysis, and relates to a method for simultaneously detecting four kinds of mycotoxins based on a multi-channel label-free electrochemical aptamer sensor, in particular to aflatoxin B1 (AFB1), fumonisin B1 (FB1), Sensitive, low-cost detection of ochratoxin A (OTA), zearalenone (ZEN). Background technique [0002] Mycotoxins are secondary metabolites of fungi (Aspergillus, Penicillium and Fusarium), mainly produced during agricultural harvesting, storage and transportation. Although more than 300 types of mycotoxins have been discovered, only a few types of mycotoxins such as aflatoxins, ochratoxins, fumonisins, and zearalenone have In crops, it has attracted people's attention. More seriously, there have been a large number of reports proving that synergistic or additive side effects between mycotoxins multiply toxicity. AFB1 and OTA usually co-exist in moldy wheat and corn, and due to their syn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327G01N27/06
Inventor 钱静崔海宁王成全王坤
Owner JIANGSU UNIV
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