Breeding method for rapidly transforming purple flowering Chinese cabbage gene to flowering Chinese cabbage

Abstract

The invention discloses a breeding method for rapidly transforming a purple flowering Chinese cabbage gene to a flowering Chinese cabbage, which comprises the following steps: (1) after respectively sowing the flowering Chinese cabbage and the purple flowering Chinese cabbage, carrying out light-dark alternate culture on the flowering Chinese cabbage at a constant temperature of 25 DEG C; the purple flowering Chinese cabbage is subjected to light-dark alternate culture under the variable-temperature condition of 25 DEG C / 10 DEG C; after culturing for 20-30 days, respectively applying a water-soluble compound fertilizer; (2) when the flowering cabbage and the purple flowering cabbage are bolted and bloomed, hybridizing by taking the purple flowering cabbage as a female parent and the flowering cabbage as a male parent to obtain F1 hybrid seeds; (3) performing peel cutting treatment on the F1 hybrid seeds, sowing the seeds, culturing the seeds according to the Chinese flowering cabbage culture mode in the step (1), and performing backcrossing on the F1 plants and Chinese flowering cabbages when the F1 plants bloom to obtain BC1 seeds; and (4) repeating the step (3) to carry out continuous multi-generation backcross. According to the method, the first generation can be bred within 1.5 months, the breeding time is greatly shortened, a large amount of labor and land cost is saved, and annual breeding of the flowering cabbages in an artificial controllable environment is achieved.

Description

technical field [0001] The invention belongs to the technical field of breeding, and in particular relates to a breeding method for quickly transferring the laver stalk gene to Chinese cabbage. Background technique [0002] Caixin, also known as cabbage sprouts, is a biennial herbaceous plant in the Brassica genus Brassica species of the Brassicaceae family. It takes flower sprouts as edible products and is mainly distributed in South China. Production. Laver sprouts are a specialty vegetable in my country. They originated in the middle of the Yangtze River Basin, Changsha, Xiangtan, Hunan, Wuhan, Hubei, and Chengdu, Sichuan. They have a long history of cultivation, and require a certain low temperature for bolting and flowering. Interspecific hybridization is an important means to expand genetic germplasm resources and create new species and materials. Because Chinese cabbage and laver stalks have different temperature requirements for bolting and flowering, the flowering...

AI Technical Summary

Problems solved by technology

[0003] Aiming at the above-mentioned prior art, the present invention provides a breeding method for quickly transferring genes from laver stalks to Chinese cabbage, so as to solve the problem of the failure of the flowering period of Chinese cabbage and laver stalks, the difficulty of using the excellent genes of laver stalks to improve Chinese cabbage, and the time for breeding. long wait question

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