Culture method for producing adventitious buds through one-step induction of Blumea balsamifera root cell differentiation

A technology of cell differentiation and culture method, which is applied in the field of one-step inducing the differentiation of Aina root cells to produce adventitious buds, can solve the problems of lack, cumbersome process, interference with mutagenesis or genetic transformation results, etc., to improve accuracy and save money. Consumables, better cultivation effect

Active Publication Date: 2022-04-22
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Claims
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Problems solved by technology

Usually artificial mutagenesis is carried out at the callus stage, but because some cells that have not been successfully mutated may also differentiate to form adventitious buds, there is also the problem of chimerism in this process
In addition, this technique needs to go through two steps, prepare at least two kinds of medium, inoculate at least twice, and cultivate at ...

Method used

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  • Culture method for producing adventitious buds through one-step induction of Blumea balsamifera root cell differentiation
  • Culture method for producing adventitious buds through one-step induction of Blumea balsamifera root cell differentiation

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Embodiment 1

[0035] A culture method for one-step induction of Aina root cell differentiation to produce adventitious buds, comprising the following steps:

[0036] S1. Get the aseptic seedlings of Ai Naxiang, get the main root and lateral root, cut the root into sections, each section is 1-4cm long, sterilize, and obtain the root section; After routine sterilization, aseptic sowing is carried out, and it is obtained by culturing for 12 days; the sterile sowing medium is 1 / 2MS as the basal medium, and 0.01-0.5mg / L NAA, 20-50g / L sucrose and 3~7g / L agar;

[0037] S2. Using MS as the basal medium, add 0.05mg / L NAA, 0.1mg / L 6-BA, 30g / L sucrose, 6g / L agar, 10mg / L vitamin C and 10mg / L polyethylene to the basal medium Pyrrolidone, to obtain adventitious bud induction medium;

[0038] S3. Inoculate the root segments of step S1 into the adventitious bud induction medium prepared in step S2, cultivate them for 20 days at 25-28°C, and use the adventitious bud induction medium to cultivate segmente...

Embodiment 2

[0040] A culture method for one-step induction of Aina root cell differentiation to produce adventitious buds, comprising the following steps:

[0041] S1. Choose healthy plants free from diseases and insect pests. After irrigating and disinfecting the soil for 3 days, dig out the root tissue of Ainaxiang without damaging the root epidermis, carefully remove the surface soil particles with a brush, rinse 3 times with tap water, and rinse 3 times with sterile water After routine sterilization in the ultra-clean bench, divide the root into sections, each section is 3-6cm long

[0042] S2. Take 1 / 2MS as the basal medium, add 0.025mg / L NAA, 0.1mg / L 6-BA, 10g / L sucrose and 6g / L agar in the basal medium to obtain adventitious bud induction medium;

[0043] S3. Inoculate the root segments of step S1 into the adventitious bud induction medium prepared in step S2, cultivate them at 25-28°C for 20 days, and use the adventitious bud induction medium to cultivate segmented root tissues to...

Embodiment 3

[0045] A culture method for one-step induction of Aina root cell differentiation to produce adventitious buds, comprising the following steps:

[0046] S1. Get Ainaxiang adventitious root segmentation, each section is 25cm long, sterilize, and obtain root segment; Described adventitious root is that explant is inoculated into adventitious root induction medium and differentiates and cultivates 30 days gain; Described adventitious root induction medium is with 1 / 2MS is the basal medium, in the basal medium, add 0.01~0.5mg / L NAA, 20~50g / L sucrose and 3~7g / L agar;

[0047] S2. prepare adventitious bud induction medium with MS as basal medium, add 0.1mg / LNAA, 2.0mg / L 6-BA, 70g / L sucrose, 8g / L agar, 10mg / L vitamin C and 10mg / L polyvinylpyrrolidone;

[0048] S3. Inoculate the root segments of step S1 into the adventitious bud induction medium prepared in step S2, cultivate them at 25-28°C for 90 days, and use the adventitious bud induction medium to cultivate segmented adventitious ...

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Abstract

The invention provides a culture method for producing adventitious buds through one-step induction of Blumea balsamifera root cell differentiation, which comprises the following steps: selecting a root segment of Blumea balsamifera as an explant for culture, designing an adventitious bud induction culture medium, and inducing the explant to directly differentiate the adventitious buds only by using the culture medium; according to the method, the blank of using the blumea balsamifera root as the explant is filled, and the effective explant source of the blumea balsamifera in the propagation process is increased. Besides, the root cells of the blumea balsamifera are directly differentiated to form the adventitious buds, and the process of forming calluses is not needed, so that the technical links are simplified, the variation of regenerated buds and the generation of chimeras are reduced, the accuracy of directional mutagenesis or genetic transformation of the blumea balsamifera is improved, and the method can be applied to breeding of the blumea balsamifera and can be applied to breeding of the blumea balsamifera. And time and labor cost can be remarkably saved, and the working efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a culture method for one-step induction of Aina radix root cell differentiation to produce adventitious buds. Background technique [0002] Ainaxiang (Blumea balsamifera (L.) DC.) is a perennial herb of the genus Ainaxiang in the family Asteraceae. It is distributed in Yunnan, Guizhou, Hainan, Taiwan, and other places in my country. It is the only source of Chinese medicine Ai Pian (L-borneol) Plants, whose extracts are the main raw materials of various Chinese patent medicines, are widely used in food, cosmeceutical and other market fields, and have good market prospects. [0003] For a long time, the planting technology of Ainaxiang has been relatively backward, and the production is mainly based on seed propagation and root division propagation. Seed propagation belongs to sexual reproduction, and the disadvantage is that the emergence rate is low, and the traits ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/005A01H6/14A01H5/12
Inventor 陈晓鹭于福来肖永锋罗沁黎玉兰陈振夏陈英桦于平元超王丹官玲亮张影波黄梅谢小丽王凯
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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