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Plasma miRNA marker related to nontuberculous pneumonia and application thereof

A non-tuberculosis and marker technology, applied in the field of biomedicine, to achieve the effects of high accuracy and sensitivity, high detection rate, simple and fast detection

Active Publication Date: 2022-04-22
SHENZHEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of this application is to provide a plasma miRNA marker related to non-tuberculous pneumonia and its application, aiming to solve the problem that there is no relevant plasma miRNA marker in the prior art that can accurately and quickly assess the risk of non-tuberculous pneumonia. The problem of making predictions

Method used

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  • Plasma miRNA marker related to nontuberculous pneumonia and application thereof
  • Plasma miRNA marker related to nontuberculous pneumonia and application thereof
  • Plasma miRNA marker related to nontuberculous pneumonia and application thereof

Examples

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Embodiment 1

[0094] An application of a miRNA marker related to non-tuberculous pneumonia in the preparation of products for the early diagnosis of non-tuberculous pneumonia, wherein the product is selected from a system for predicting the risk of non-tuberculous pneumonia, and the system includes:

[0095] (1) Data acquisition unit: used to perform real-time fluorescence quantitative reaction based on Direct S-Poly(T)Plus on the sample, and obtain the Ct value obtained by real-time fluorescence quantitative reaction of plasma miRNA markers in the sample.

[0096] The specific steps are as follows: including:

[0097] ① Collection and processing of plasma samples from subjects

[0098] A total of 146 patients with active pulmonary tuberculosis and 139 patients with non-tuberculous pneumonia were included in this embodiment. Among them, the first batch of samples was used for miRNA marker screening, including 79 cases of active pulmonary tuberculosis patients and 60 cases of non-tuberculou...

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Abstract

The invention relates to the technical field of biomedicine, in particular to a plasma miRNA marker related to non-tuberculous pneumonia and application of the plasma miRNA marker related to the non-tuberculous pneumonia, and provides the plasma miRNA marker related to the non-tuberculous pneumonia, and the plasma miRNA marker comprises one or more of miR-223-3p, miR-23b-3p, miR-4532, miR-378c, let-7c-5p, miR-15b-3p, miR-665, miR-143-3p, miR-1332-3p, miR-10a-5p and miR-335-5p.

Description

technical field [0001] The application belongs to the field of biomedical technology, and in particular relates to a plasma miRNA marker related to non-tuberculous pneumonia and its application. Background technique [0002] Generally speaking, pneumonia refers to the infectious verification of alveoli, distal airways, and lung interstitium, which can be caused by factors such as bacteria, viruses, and other pathogens. Among them, bacterial pneumonia and viral pneumonia are the most common; among them, Common bacteria include Streptococcus pneumoniae, Staphylococcus, Haemophilus influenzae, etc., viral pneumonia, common viruses such as respiratory syncytial virus, influenza virus, parainfluenza virus, adenovirus, etc. Among them, active pulmonary tuberculosis is a relatively common infectious disease, mainly a chronic infectious disease caused by Mycobacterium tuberculosis infection, anti-tuberculosis medication and treatment cycle are quite different from non-tuberculous pn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6851C12N15/11G16B40/20G16B40/30G06K9/62
CPCC12Q1/6883C12Q1/6851G16B40/20G16B40/30C12Q2600/158C12Q2600/178G06F18/23G06F18/24
Inventor 陈心春张惠华胡云龙蔡毅
Owner SHENZHEN UNIV
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