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Composite carbonized polymer dot as well as preparation method and application thereof

A technology of polymer dots and carbonization, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve problems such as unsatisfactory, false positive rate, complex operation detection time, etc.

Pending Publication Date: 2022-05-03
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the results obtained by these detection methods are not very satisfactory in practical applications, because they have various disadvantages, such as false positive rate, instrument cost, complex operation and long detection time, etc.

Method used

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  • Composite carbonized polymer dot as well as preparation method and application thereof
  • Composite carbonized polymer dot as well as preparation method and application thereof
  • Composite carbonized polymer dot as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1 provides a kind of composite carbonized polymer point, and the preparation method is as follows:

[0056] S1. Dissolve 0.054 g of o-phenylenediamine in 10 mL of deionized water, then add 50 μL (0.725 mM) of HNO 3 , after stirring at room temperature for 5 minutes, a mixed reaction solution was formed;

[0057] S2. Transfer the mixed reaction solution into a polytetrafluoroethylene-lined reaction kettle, and heat in an oven at 200°C overnight, and the reaction mixture is cooled to room temperature.

[0058] S3. After filtering the solution obtained in step S2 with a 0.22 μm polyethersulfone membrane to remove large particles, dialyze the deionized water in a 500D dialysis bag to obtain a carbonized polymer dot solution, and freeze-dry the carbonized polymer dot solution for storage .

[0059] Add 3.5 mg EDC and 5 mg NHS to the 1 mg / mL solution of carbonized polymer dots, then stir for 30 min. Then 100 μL of 1 mg / mL S100-β peptide was added to the mixture ...

Embodiment 2

[0061] Example 2 provides a composite carbonized polymer dot. The preparation method and raw materials are the same as those in Example 1, the difference being that the concentration of S100-β peptide is 0.1 mg / mL.

Embodiment 3

[0063] Embodiment 3 provides a composite carbonized polymer dot. The preparation method and raw materials are the same as those in Embodiment 1, the difference being that the concentration of S100-β peptide is 0.5 mg / mL.

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Abstract

The invention provides a composite carbonized polymer dot as well as a preparation method and application thereof. The composite carbonized polymer dot comprises a carbonized polymer dot and S100 calcium binding protein, and the carbonized polymer dot and the S100 calcium binding protein are connected through an amido bond. The composite carbonized polymer dots (pep-CPDs) can be used for rapidly and sensitively detecting the mTBI biomarker S100-beta, and in-vivo and in-vitro imaging is realized. A lactic dehydrogenase (LDH) test, an MTT test, cell imaging and a mouse acute toxicity test prove that the pep-CPDs are safe and low in cytotoxicity. By detecting S100-beta in a blood sample of an mTBI patient, the reliability of detecting an actual sample by pep-CPDs is proved.

Description

technical field [0001] The invention belongs to the technical field of carbon nanomaterials, in particular to a composite carbonized polymer point and its preparation method and application. Background technique [0002] Mild traumatic brain injury (mild traumatic brain injury, mTBI) is the most common type of traumatic brain injury. The World Health Organization (WHO) defines mTBI as a blow to the head that results in acute brain disturbance, usually resulting in physical, cognitive and / or emotional symptoms. Frequently reported long-term complications in patients with mTBI include increased anxiety, irritability, depression, and emotional lability, which have emerged as a common but underlying clinical problem. mTBI is often difficult to accurately identify in its early stages due to atypical radiologic features and imaging findings, mild symptoms, and lack of more reliable biomarkers and assays. These problems affect the authority and impartiality of forensic identifica...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07K1/13C09K11/65C09K11/02G01N21/64
CPCC07K14/4728C09K11/65C09K11/025G01N21/6428G01N2021/6432G01N2021/6417
Inventor 吕奔石坚李杏梅蔡继峰李娜
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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