Burnet dwarf extract and its application in the preparation of cosmetics

A technology of dwarf burnet and extract, which is applied in the directions of cosmetics, cosmetic preparations, dressing preparations, etc., to achieve the effects of improving resource utilization value, utilization function and added value, and good safety.

Active Publication Date: 2018-05-18
云南西草资源开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Except for Naxi medicine, the use of Burnet japonicus in my country is relatively rare, and there is no report on the activity of Burnet japonicus or its extract in skin cosmetics or medicines.

Method used

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  • Burnet dwarf extract and its application in the preparation of cosmetics
  • Burnet dwarf extract and its application in the preparation of cosmetics
  • Burnet dwarf extract and its application in the preparation of cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of Burnet dwarf extract:

[0022] Weigh 12 g of the whole herb sample of Sanguisorba filiformis (Hook.f.) Hand.-Mazz., grind the sample into powder, cold-soak with 25 times the amount of 85% ethanol (361-362mL) for 24h, and then use The filtrate was collected by Buchner funnel and repeated 3 times. The filtrate was distilled under reduced pressure with a rotary evaporator, the filtrate was concentrated, and the concentrated filtrate was dried at low temperature to obtain a crude extract. The crude extract was adsorbed and decolorized by macroporous resin (D101), and eluted with 95% ethanol, the eluate was concentrated, evaporated to dryness, and freeze-dried at low temperature to obtain 3.3 g of Burnet burnet herb extract, with an extraction rate of 27.5%.

[0023] Alternatively, weigh 12 g of the root sample of Sanguisorba filiformis (Hook.f.) Hand.-Mazz., grind the sample into powder, cold soak with 20 times the amount of 95% ethanol for 20 hours, and the...

Embodiment 2

[0025] Cytotoxicity test of Burnet dwarf extract: Determination of CC of samples by MTS colorimetry 50 (50%cytotoxicconcentration), that is, the drug concentration when producing toxicity to 50% of the cells, thereby determining the safe sample concentration for the following activity experiments.

[0026] Experimental method: on a 96-well cell culture plate, mix B16 cells with different concentrations of the drug solution to be tested, set 3 replicate wells, and set a blank control without drug at the same time, 37 ° C, 5% CO 2 After culturing for 24 hours, the cytotoxicity was detected by the MTS colorimetric method, and the OD value was measured by a microplate reader at a wavelength of 490 nm. Calculated to get CC 50 value.

[0027] Cell survival rate (%) = experimental well OD 490nm / blank well OD 490nm ×100%

[0028] Experimental results: the cell survival rate of the whole herb extract of Burnet burnet is 87.343%, which belongs to the normal level. It can be clearl...

Embodiment 3

[0030] DPPH free radical scavenging experiment: mix the whole herb or root extract of the drug to be tested with DPPH (final concentration: 100 μM), set up 3 repeated wells, and set up a blank control without drug and a positive control of Trolox at the same time, 30°C, 1h, measure the OD value with a microplate reader, and the detection wavelength is 515nm. Calculate the antioxidant rate.

[0031] Antioxidant rate (%) = (1‐experimental hole OD 515nm / blank well OD 515nm )×100%

[0032] Experimental results: In the experiment, the whole plant or root extract of Burnet burnet exhibited significant DPPH free radical scavenging ability, and according to the concentration gradient, it showed a good dose-effect relationship (see figure 1 ), can be used as a good natural plant source of cosmetic activity with anti-wrinkle or sunscreen potential.

[0033] The present invention provides topical compositions having natural botanical ingredients or mixtures of natural botanical ingr...

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Abstract

The invention discloses Sanguisorba officinalis L. extract, a cosmetic additive adopting the Sanguisorba officinalis L. extract for realizing skin whitening, moisturizing and antioxidant activity and an application of the Sanguisorba officinalis L. extract in cosmetics. The Sanguisorba officinalis L. extract is an ethanol extract of whole herb or root part of Sanguisorba officinalis L. and the extract has good DPPH free radical clearing capacity and is a good natural antioxidant. The Sanguisorba officinalis L. extract has excellent skin cosmetic application potential in the aspects of wrinkle resisting, skin whitening, sunscreen and the like in terms of skin care of a human body; the Naxi traditional medicinal plant, namely the Sanguisorba officinalis L. is effectively utilized, the Sanguisorba officinalis L. extract with high skin cosmetic activity is obtained, and the extract can be used for preparing a good antioxidant, has excellent functions of wrinkle resisting, skin whitening, sunscreen and the like and is good raw material for natural plant-based cosmetics.

Description

technical field [0001] The invention belongs to the technical field of cosmetics and medicine, and in particular relates to a natural active substance extracted from Burnet dwarf Burnet, a medicinal plant handed down by the Naxi nationality, a cosmetic or medicine using it as an active ingredient, and Burnet dwarf The application of elm extract in the preparation of various skin cosmetics such as moisturizing, skin rejuvenation, anti-wrinkle, sunscreen, and whitening, as well as in the preparation of drugs for the treatment or prevention of skin diseases technical background [0002] Dwarf Burnet (Sanguisorba filiformis (Hook.f.) Hand.-Mazz.) is a plant of the Rosaceae Burnet genus, a perennial herb with a cylindrical root and a brown surface. The basal leaves are pinnate compound leaves with 3-5 leaflets Yes, the petiole is smooth, the small leaves have short stalks and are rarely sessile, broadly ovate or nearly round, the flowers are unisexual, monoecious, and the inflore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61K36/739A61Q19/02A61Q19/08A61Q17/04A61Q19/00A61P39/06A61P17/18A61P17/00
CPCA61K8/97A61K9/0043A61K9/08A61K9/12A61K9/2013A61K9/2031A61K9/4858A61K36/739A61K2236/333A61K2236/51A61K2236/53A61K2236/55A61Q17/04A61Q19/00A61Q19/02A61Q19/08
Inventor 杨立新许刚刘爱忠裴盛基
Owner 云南西草资源开发有限公司
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