Device and method for continuously monitoring cell growth rate

A growth rate and cell technology, applied in the field of microbial detection, can solve problems such as increasing the workload of laboratory workers, reducing the volume of culture solution, affecting the growth of microbial cells, etc., to prevent cell contamination and loss of culture solution, increase service life and stability. performance, improved efficiency, and experimental precision

Pending Publication Date: 2022-05-03
NANJING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

This method requires the experimenter to regularly and repeatedly open the bottle to take samples for testing, which is easy to introduce pollution and increase the workload of the experimenter
Moreover, the room temperature and the temperature of the culture conditions are different. Repeated sampling will affect the growth of microbial cells, and multiple sampling will cause the volume of the culture medium to decrease, resulting in inaccurate test data and unable to truly reflect the growth status of microbial cells.
And for microbial cells that need to be cultured under anaerobic or other special conditions, it is very difficult to measure the growth rate of microbial cells by manual sampling

Method used

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  • Device and method for continuously monitoring cell growth rate
  • Device and method for continuously monitoring cell growth rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] Step 1, prepare the LB liquid medium required for the growth of E.coli DH5α, put it into six 250mL colorless transparent Erlenmeyer flasks with paired grooves on the bottom, the liquid volume is 100mL, seal and sterilize at 121°C 20min;

[0025] Step 2, after the sterilized medium is cooled to room temperature, inoculate the single colony of E.coli DH5α on the solid LB plate into six Erlenmeyer flasks;

[0026] Step 3, insert the sensor in the bottom grooves of the six Erlenmeyer flasks in step 2;

[0027] Step 4, put the Erlenmeyer flask with the sensor inserted in step 3 into the shaker, set the temperature of the shaker to 37°C, and the speed to 200rpm;

[0028] Step 5, open the software that outputs the growth curve of the bacteria, select the detection channel 1-6, set the sampling interval to 10s, and start the shaker to start the cultivation;

[0029] Step 6, transmit the turbidity data of the monitored E.coli DH5α culture solution to the computer through the w...

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Abstract

The invention discloses a device and a method for continuously monitoring a cell growth rate. The device comprises a culture bottle with a groove in the bottom, a sensor inserted into the groove and a sensor fixing device, the turbidity of a cell culture fluid in the culture bottle is measured at the bottom of the culture bottle through the sensor in the groove, the turbidity value of the cell culture fluid is obtained through a data processing and data output module, and a growth curve is drawn. The growth rate of the cells is dynamically monitored in real time. When the cell growth rate is monitored, a culture bottle does not need to be opened for sampling, the cell growth rate can be rapidly, accurately and continuously monitored, a growth curve can be drawn, the workload of an experimenter is reduced, and the experiment accuracy is improved.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and relates to a device and a method for continuously monitoring cell growth rate. Background technique [0002] The growth curve of microbial cells is a curve that reflects the change of microbial cells during the culture period, drawn with the culture time as the abscissa and the change in the number of microbial cells as the ordinate when the microbial cells are cultured under suitable conditions. For example, the bacterial growth curve reflects the different stages of bacterial growth: lag phase, logarithmic phase, stable phase, and decay phase, which can reflect the number, growth status, and metabolism of bacteria. The growth curve of microbial cells has important guiding significance in the real-time monitoring of microbial culture and fermentation process, the screening of cloned strains and the characterization of growth characteristics, microbial inhibition and drug toxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/24C12M1/00C12Q1/06
CPCC12M41/36C12M23/08C12M23/22C12M23/46C12Q1/06
Inventor 张建法孙夏青
Owner NANJING UNIV OF SCI & TECH
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