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Gamma-polyglutamic acid high-producing strain and application thereof

A technology of polyglutamic acid and strains, applied to bacteria, adding compounds to stimulate growth, microorganisms, etc., can solve unseen problems, and achieve the effects of low fermentation cost, production cost reduction, and high yield

Active Publication Date: 2022-05-10
天津北洋百川生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that the yield of γ-polyglutamic acid fermented with molasses reached 32.7g / L, which not only obtained the target product, but also improved the utilization rate of resources, but there is no case of practical application in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] In this example, the specific process of using Bacillus licheniformis to ferment γ-polyglutamic acid is as follows: take Bacillus licheniformis (Bacillus licheniformis) BCSW-11052 and transfer it to the slant medium and cultivate it at a constant temperature at 37°C for 8 hours, and select the well-growing The slant seeds were inoculated into the seed medium using an inoculation loop, cultivated at 37°C and 220rpm for 12 hours, then took the culture solution and inoculated it into a shaker flask according to the inoculum size of 10%, and the shaker flask contained 50mL of fermentation medium, and then Cultivate for 72 hours at 37°C and 220 rpm, and detect the content of γ-polyglutamic acid in the culture solution. The results show that the output of γ-polyglutamic acid in this example is 33.5g / L.

[0056] The components and contents of the seed medium used are as follows: 20 g of glucose, 5 g of yeast extract, 8 g of tryptone, 0.4 g of magnesium sulfate, 0.4 g of dipota...

Embodiment 2

[0058] In this example, the specific process of using Bacillus licheniformis to ferment γ-polyglutamic acid is as follows: take Bacillus licheniformis (Bacillus licheniformis) BCSW-11052 and transfer it to the slant medium and cultivate it at a constant temperature at 37°C for 8 hours, and select the well-growing The slant seeds were inoculated into the seed medium with an inoculation loop, and cultivated at 37°C for 12 hours at a speed of 220rpm, and then the culture solution was inoculated into a shaker flask according to 10% of the inoculation amount. Cultivate at 220 rpm for 72 hours, and detect the content of γ-polyglutamic acid in the culture solution. The results showed that the yield of gamma-polyglutamic acid was 62.9g / L in this example.

[0059] The components and contents of the seed medium used were as follows: 30 g of glucose, 7 g of yeast extract, 10 g of tryptone, 0.5 g of magnesium sulfate, 0.5 g of dipotassium hydrogen phosphate, the solvent was water, and ste...

Embodiment 3

[0061] In this example, the specific process of using Bacillus licheniformis to ferment γ-polyglutamic acid is as follows: take Bacillus licheniformis (Bacillus licheniformis) BCSW-11052 and transfer it to the slant medium and cultivate it at a constant temperature at 37°C for 8 hours, and select the well-growing The slant seeds were inoculated into the seed medium using an inoculation loop, cultivated at 37°C and 220rpm for 12 hours, then took the culture solution and inoculated it into a shaker flask according to the inoculum size of 10%, and the shaker flask contained 50mL of fermentation medium, and then Cultivate for 72 hours at 37°C and 220 rpm, and detect the content of γ-polyglutamic acid in the culture solution. The results show that the output of γ-polyglutamic acid in this example is 67.5g / L.

[0062] The components and contents of the seed medium used were as follows: 30 g of glucose, 7 g of yeast extract, 10 g of tryptone, 0.5 g of magnesium sulfate, 0.5 g of dipo...

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PUM

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Abstract

The invention provides a gamma-polyglutamic acid high-producing strain. The biological preservation number of the gamma-polyglutamic acid high-producing strain is CGMCC (China General Microbiological Culture Collection Center) The bacillus licheniformis strain is named as BCSW-11052, and belongs to bacillus licheniformis (Bacillus licheniformis). The invention also provides application of the gamma-polyglutamic acid high-producing strain as a gamma-polyglutamic acid fermentation strain, and a method for fermenting gamma-polyglutamic acid by using the gamma-polyglutamic acid high-producing strain. The high-yield strain BCSW-11052 disclosed by the invention has the beneficial effects that by utilizing the high-yield strain BCSW-11052, cane molasses with low price is used as a carbon source, and a small amount of yeast extract and ferrous sulfate are additionally added, so that the yield of gamma-polyglutamic acid is greatly increased, and the production cost is reduced. The yield of the gamma-polyglutamic acid can reach 80 g / L or above, and compared with the prior art, a great breakthrough is made.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and provides a high-yielding gamma-polyglutamic acid strain and a method for fermenting gamma-polyglutamic acid using it. Background technique [0002] Polyglutamic acid is polymerized by D-glutamic acid and L-glutamic acid through amide bonds, and glutamic acid monomers polymerized through γ-amide bonds are called γ-polyglutamic acid. As a new type of multifunctional biological product, γ-polyglutamic acid has the characteristics of non-toxic, harmless, edible, easy to degrade and water retention, and has strong applications in many fields such as agricultural production, medicine and food prospect. In agriculture, it is mostly used as a moisturizer and fertilizer synergist to help plants grow with sufficient water and promote plants to absorb fertilizers better. In the pharmaceutical industry, it is mainly used as a drug carrier and tissue engineering scaffold; in food, γ-polyglutamic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12P13/02C12R1/10
CPCC12N1/20C12N1/38C12P13/02
Inventor 乔长晟李祥松赵廷彬郭利飞盖丽丰
Owner 天津北洋百川生物技术有限公司
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