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Non-programmed cell freezing medium and application method thereof

An application method and cryopreservation technology, applied in the field of cell and bioengineering, can solve the problems of complex serum components, time-consuming and laborious, large batch differences, etc., and achieve the effects of process stability, guaranteed stability, and small batch differences.

Pending Publication Date: 2022-05-13
广州松鼠生物科技有限公司
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Problems solved by technology

[0003] The traditional cell cryopreservation solution is a mixture of culture medium, serum and DMSO in a certain proportion. Due to the shortcomings of complex serum components and large batch differences, its application has limitations, and the cryopreservation method of programmed cooling is required. Time-consuming

Method used

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  • Non-programmed cell freezing medium and application method thereof
  • Non-programmed cell freezing medium and application method thereof

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Embodiment Construction

[0040] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them; based on The embodiments of the present invention and all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0041] It should be noted that the words "front", "rear", "left", "right", "upper" and "lower" used in the following description refer to the directions in the drawings, and the words "inner" and "outer ” refer to directions towards or away from the geometric center of a particular part, respectively.

[0042] A non-programmed cell freezing solution, each 100mL of the cell freezing solution includes 0.01g of sodium L ascorbate, 0.0001g of D-biot...

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Abstract

The invention relates to the technical field of bioengineering, and discloses a non-programmed cell freezing medium and an application method thereof. Every 100 mL of the cell freezing medium is prepared from 0.01 g of L sodium ascorbate, 0.0001 g of D-biotin, 0.001 g of choline chloride, 0.001 g to 0.01 g of folic acid, 1 to 20 g of inositol, 0.0005 to 0.005 g of nicotinamide, 0.0001 to 0.005 g of vitamin B12, 0.0005 to 0.005 g of D-calcium pantothenate, 0.0005 to 0.005 g of pyridoxal hydrochloride, 0.00001 to 0.0001 g of pyridoxine hydrochloride, 0.00005 to 0.0005 g of riboflavin, 0.0005 to 0.005 g of ammonium sulfate hydrochloride, 0.0001 to 0.005 g of hypoxanthine, 10 to 100 g of cane sugar, 20 to 200 g of propylene glycol, 0.5 to 5 g of sodium carboxymethyl cellulose and the balance of water.

Description

technical field [0001] The invention relates to the technical field of cells and bioengineering, in particular to a non-programmed cell cryopreservation solution and an application method thereof. Background technique [0002] Cell cryopreservation is one of the important links in biological research. It freezes artificially cultured cells, prevents cell degradation caused by cell subculture, prevents bacterial contamination in subculture, and solves a series of problems in the process of maintaining subculture. Questions matter. [0003] The traditional cell cryopreservation solution is a mixture of culture medium, serum and DMSO in a certain proportion. Due to the shortcomings of complex serum components and large batch differences, its application has limitations, and the cryopreservation method of programmed cooling is required. Time-consuming. Contents of the invention [0004] In view of the above situation, in order to make up for the above-mentioned existing defe...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 严广华
Owner 广州松鼠生物科技有限公司
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