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Hepatocyte refrigerating fluid

A technology of hepatocytes and frozen liquid, applied in the field of biomedicine, can solve the problem of low survival rate of hepatocytes, and achieve the effect of promoting the regeneration of hepatocytes, achieving the best effect and avoiding the risk of toxicity.

Inactive Publication Date: 2022-03-01
苏州熠品质量技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the survival rate of the existing cryogenic fluids for hepatocyte resuscitation is not high, therefore, it is urgent to provide a hepatocyte freezing fluid with a high survival rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 A kind of liver cell cryogenic liquid

[0030] (1) 60% v / v basal medium (containing 1% v / v antibiotics);

[0031] (2) 15% v / v glycerol;

[0032] (3) The active ingredient of 25% v / v; the active ingredient contains bovine albumin 0.3g / mL, glucose 78% v / v, 18AA compound amino acid 10% v / v, hepatocyte growth factor 4v / v and Chinese medicine extract 8% v / v; the volume mass ratio of Chinese medicine extract is 3mL: 1mg of Lycium barbarum extract and Angelica sinensis extract.

Embodiment 2

[0033] Embodiment 2 A kind of hepatocyte freezing liquid

[0034] (1) 70% v / v basal medium (containing 1.5% v / v antibiotics);

[0035] (2) 20% v / v glycerin;

[0036] (3) 10% v / v active ingredient; the active ingredient contains bovine albumin 0.5g / mL, glucose 70% v / v, 18AA compound amino acid 15% v / v, hepatocyte growth factor 1% v / v and Chinese medicine extract 10% v / v; the volume mass ratio of Chinese medicine extract is 2mL: 1mg of Lycium barbarum extract and Angelica sinensis extract.

Embodiment 3

[0037] Embodiment 3 A kind of hepatocyte freezing liquid

[0038] (1) 50% v / v basal medium (containing 0.8% v / v antibiotics);

[0039] (2) 10% v / v glycerin;

[0040] (3) 40% v / v active ingredient; the active ingredient contains bovine albumin 0.05g / mL, glucose 80% v / v, 18AA compound amino acid 25% v / v, hepatocyte growth factor 8% v / v and Chinese medicine extract 1% v / v; the volume mass ratio of the Chinese medicine extract is 4mL: 1mg of Lycium barbarum extract and Angelica sinensis extract.

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Abstract

The invention discloses a liver cell refrigerating fluid which comprises 50-70% v / v of a basic culture medium, 10-20% v / v of glycerol and 10-40% v / v of an active component. The cell freezing liquid does not contain DMSO, the toxicity risk to cells is avoided, main nutrient substances such as sugar, amino acid, protein and growth factors are provided for the liver cell cryopreservation process by adding the traditional Chinese medicine extract, and the recovery survival rate of the liver cells is remarkably increased.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a liver cell freezing liquid. Background technique [0002] As an effective method of preserving cells, cell cryopreservation has been widely used in the field of biology. Under normal circumstances, direct freezing of cells will produce ice crystals that will damage the cells and lead to cell death. Therefore, during the cryopreservation process of cells, it is necessary to add a cryopreservation solution to ensure the integrity and functional integrity of the cells during the cryopreservation process. [0003] The most commonly used cell freezing solution at this stage is mainly composed of culture medium, fetal bovine serum (FBS) and dimethyl sulfoxide (DMSO). Among them, DMSO is antifreeze, which can improve the permeability of cell membrane to water and reduce cell damage caused by ice crystal formation. However, with people's pursuit of safety and non-toxicity, and DM...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 汪子霞王璇
Owner 苏州熠品质量技术服务有限公司
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