A kind of serum-free, protein-free and DMSO-free cell cryopreservation solution and application thereof

Abstract

The invention discloses a serum-free, protein-free and DMSO-free cell cryopreservation solution and application thereof. The invention first discloses a serum-free, protein-free and DMSO-free cell cryopreservation solution, which includes the following components: glucose injection, compound amino acid injection, glycerin, potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride , Sucrose, Hetastarch, ATP-Disodium Magnesium Chloride and Water. The invention further discloses the application of the above-mentioned cell cryopreservation solution in freezing cells. The composition of the cell cryopreservation solution of the present invention is clear, the batch is stable, and meets the requirements of industrial transformation. In addition, the cell cryopreservation solution of the present invention is serum-free, protein-free, and DMSO-free, and can stably maintain the activity and dryness of cells for a long time when cells are frozen. Sexuality requirements and validity requirements.

Description

technical field [0001] The invention relates to the field of cell cryopreservation. More specifically, it relates to a serum-free, protein-free and DMSO-free cell cryopreservation solution and its application. Background technique [0002] At present, cell therapy is more and more widely used in clinical practice. In particular, stem cell therapy has great advantages and broad development prospects in the treatment of degenerative, traumatic, genetic defect and other diseases (Ella Buzhor, Lucy, Leshansky, Jacob Blumenthal, et al. Cell-based therapy approaches: the hope for incurable diseases. Regenerative Medicine. (2014) 9(5), 649–672). Efficient and safe cell preparations are the key factors in cell therapy. Traditional cell preparations consist of a mixture of living cells and an electrolyte solution (such as Bomalis A). After the preparation is completed, it generally needs to be reinfused within 12 hours, and the cell viability will decrease after 12 hours, which w...

AI Technical Summary

Problems solved by technology

Although the incidence of toxic and side effects can be reduced by washing, the washing process will lead to a significant reduction in the number of cells, especially the stem cells themselves, and further loss may lead to treatment failure due to insufficient transplanted cells

In addition, washing the cells after recovery is not only cumbersome but also increases the risk of cell contamination

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