Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fusion gene, protein coded by fusion gene and application of fusion gene in fish iridovirus oral vaccine

A technology of fusion gene and fusion protein, applied in the directions of virus, vaccine, application, etc., to achieve the effects of simple operation, increased serum specific antibody level, and increased IgM content

Active Publication Date: 2022-05-13
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The only way to prevent and treat this viral disease is through vaccines and other immune products.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fusion gene, protein coded by fusion gene and application of fusion gene in fish iridovirus oral vaccine
  • Fusion gene, protein coded by fusion gene and application of fusion gene in fish iridovirus oral vaccine
  • Fusion gene, protein coded by fusion gene and application of fusion gene in fish iridovirus oral vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0061] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific implementations described in this specification are only for explaining the present invention, not for limiting the present invention.

[0062] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial channels.

[0063] Reagents and materials

[0064] The largemouth bass used in the following examples are all healthy largemouth bass (body length 8.5 ± 0.7cm, body weight 15.3 ± 2.5g) from Guangdong Province Aquatic Breeding Farm.

[0065] Largemouth bass iridescent virus, pNZ8148 plasmid, HRP-labeled sheep anti-mouse antibody, and mouse anti-grass carp IgM antibody were all preserved in the Aquatic Disease and Immunity Laboratory...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a fusion gene, a protein coded by the fusion gene and application of the fusion gene in fish iridovirus oral vaccines. The fusion gene contains a micropterus salmoides iridovirus capsid protein MCP antigen optimization gene, a connection sequence and a tag sequence. The recombinant lactococcus lactis NZ9000 pNZ8148-MCP prepared on the basis of the recombinant lactococcus lactis NZ9000 pNZ8148-MCP is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20211160. After the oral vaccine prepared from the recombinant lactococcus lactis is used for immunization, the serum specific antibody level of largemouth bass can be remarkably improved, the IgM content in the kidney can also be remarkably improved after the oral vaccine is used for immunization, the relative immune protection rate can reach 60.47%, injection is not needed, oral administration can be achieved, operation is easy, and universality and applicability are high.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a fusion gene, its encoded protein and its application in fish iridescent virus oral vaccine. Background technique [0002] Largemouth bass ranavirus (LMBV), a member of the genus Ranavirus in the family Iridoviridae, is a large cytoplasmic double-stranded DNA virus. LMBV can survive in natural hosts such as amphibians and poultry, and even in fish bait, and there are multiple transmission routes. At present, there is no effective treatment drug, and it can only be prevented by scientific breeding and timely disinfection. This type of frog virus disease occurs frequently in summer, when the water temperature is 25-30°C, it will harm adult fish, and the fish infection fatality rate is high. [0003] Largemouth bass (Micropterus Salmoides), also known as California perch, is an important freshwater cultured fish species with fast growth and strong environmental adaptability. The annu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/34C12N15/62C12N15/74C12N1/21C07K19/00A61K39/12A61P31/20C12R1/46
CPCC07K14/005C12N15/74A61K39/12A61P31/20C12N2710/00022C12N2710/00034C12N2800/22C12N2800/101C07K2319/21C07K2319/00A61K2039/523A61K2039/542A61K2039/552Y02A40/81
Inventor 赖迎迢黄志斌巩华陶家发
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com