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Preparation method of triazole C

A technology of nitrogen bacteria and bacterial liquid, applied in the field of microbial fermentation technology and separation, can solve the problems of hindering the research and application of triazocin, long fermentation time of triazocin C, complicated separation and purification process, etc., and achieves high implementation power. , Simple operation, good repeatability

Pending Publication Date: 2022-05-13
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Claims
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Problems solved by technology

In summary, in the prior art, the fermentation time for the production of triazzocin C by microbial methods is longer, the fermentation yield is low, the separation and purification process is complicated and involves toxic and polluting reagents, the yield is low and the product purity obtained is not high, these All hinder the research and application of triazine

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  • Preparation method of triazole C
  • Preparation method of triazole C
  • Preparation method of triazole C

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Experimental program
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Effect test

Embodiment 1

[0027] The preparation method of embodiment 1, azocin C

[0028] Step 1. Activation of the strain

[0029] Each tube containing 3 mL of TSB medium was sterilized at 121°C for 20 minutes and then used for later use. Streptomyces aureus preserved in the glycerol tube was inoculated into the above test tube with an inoculum size of 20 uL per tube, and cultured on a shaker overnight to obtain liquid bacteria solution, the rotational speed of the shaker culture was 180 rpm / min, and the culture temperature was 30°C.

[0030] Step 2, fermentation and cultivation:

[0031] Prepare liquid ISP2 medium, and sterilize at 115°C for 20 minutes for backup. The liquid bacterial liquid obtained by activating the strain in step 1 was transferred to a 250 mL Erlenmeyer flask containing 50 mL of ISP 2 liquid medium at a volume ratio of 2%, and cultured on a shaking table to obtain a fermentation liquid. A total of 60 L was fermented, the rotational speed of the rotary shaker was 180 rpm / min, t...

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Abstract

The invention discloses a preparation method of triazole C, and belongs to the technical field of microorganisms and separation. In order to solve the problems existing in the production of the triazole C by using a microbial method in the prior art and improve the yield and the purity of the triazole C, the method disclosed by the invention is characterized in that streptomyces aureus is utilized for fermentation, and a pure triazole C product is obtained by means of methanol extraction, ethyl acetate extraction, silica gel column separation, HPLC (High Performance Liquid Chromatography) purification and the like. Compared with the prior art, the method has the advantages that the yield can be increased by 117%, the yield is increased by about 3.5 times, the purity of the obtained triazole C is larger than 95%, and the method is high in safety, low in pollution, simple in step and capable of being used for producing the triazole C.

Description

technical field [0001] The invention belongs to the field of microbial fermentation technology and separation technology, and in particular relates to a preparation method of triazine C. Background technique [0002] Triacsin C (Triacsin C), a hydroxytriazene compound produced by Streptomyces, is an inhibitor of long-chain fatty acid acyl-CoA synthetase, and has the functions of regulating lipid metabolism, lowering blood pressure, vasodilator, inhibiting Mobilization of intracellular calcium to inhibit activities such as pancreatic β-cell function. In addition, studies have shown that triazine C and its analogues also have the activity of inhibiting rotavirus replication, and are expected to be developed as anti-rotavirus infection drugs. [0003] Since triazine family compounds can only be obtained by microbial fermentation at this stage, the only article about its fermentation and purification "STUDIES ON NEW VASODILATORS, WS-1228 A AND B" reported that after 6 days of f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C07C249/00C07C251/00C12R1/485
CPCC12P13/001C07C249/00C07C251/00
Inventor 咸漠黄伟
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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