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DNA molecular logic gate based on nucleic acid hybridization

A DNA molecule and nucleic acid hybridization technology, applied in the field of DNA molecular logic gates, can solve the problems of highly complex number of DNA chains, linear superposition, increase design, operation and chain complexity, etc., and achieve the effect of simple and easy operation of experimental steps

Pending Publication Date: 2022-05-13
SHANGHAI IND TECH INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, previous DNA logic circuit architectures have two limitations
First, calculations are slow, often taking hours to calculate a simple function
Second, the circuit is highly complex for the number of DNA strands
These algorithms have a flaw: their experimental steps are linearly superimposed
However, both approaches require very much additional work on the DNA origami or gates, which increases the complexity of the design, manipulation, and chain

Method used

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  • DNA molecular logic gate based on nucleic acid hybridization
  • DNA molecular logic gate based on nucleic acid hybridization
  • DNA molecular logic gate based on nucleic acid hybridization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] 1. Introduction of AND gate:

[0144] Such as figure 1As shown, the input units are ss1 and ss2, and the conversion unit contains the substrate chain for addition operation. When only ss1(0,1) is input, the addition operation cannot occur, and no signal is output; when only ss2(1,0) is input, the addition operation cannot occur, and there is no signal output; when both ss1 and ss2 are input (1, 1), constitute the conditions of the addition operation, generate the result chain, constitute the signal output, and can amplify the signal through PCR amplification.

[0145] 1) truth table

[0146]

[0147] 2) Input strand ssDNA design: According to the design method of the substrate strand, each of the two input strands includes a substrate strand binding part (H or CS) and a connection complementary part (P), each part is 20bp; ss1( 0,1)= H 0 [0]-P 1 , ss2(1,0)= P 1 -C 1 S 0 [00].

[0148] 3) Substrate chain: A with biotin 0 Chain (A 0 =PrimerL-H 0 [0]), add...

Embodiment 2

[0171] OR gate introduction

[0172] Such as figure 2 As shown, the input units are ss3 and ss4, and the conversion unit contains the substrate chain for the addition operation. Unlike the AND gate, two connection chains are added to ensure that the addition operation can run. When only ss3(0,1) is input, an addition operation can occur and a signal output is generated; when only ss2(1,0) is input, an addition operation can occur and a signal output is generated; when ss1 and ss2 are input at the same time (1, 1), addition operations can also occur to generate signal output.

[0173] 1) truth table

[0174]

[0175]

[0176] 2) Input strand ssDNA design: According to the design method of the substrate strand, each of the two input strands includes a substrate strand binding part (H or CS) and a connecting complementary part (P), ss3(0,1)= H 0 [0]-P 1 , ss4(1,0)= H 0 [1]-P 0 .

[0177] 3) Substrate chain: 2 kinds of A with different assignment of biotin 0 chain...

Embodiment 3

[0212] NAND gate introduction

[0213] Such as image 3 As shown, the input units are ss5 and ss6, the conversion unit includes the substrate chain for addition operation, and two B0 chains (B0=0, B0=1) are added to ensure that the addition operation can run. When only ss5(0,1) is input, ss5 is combined with B0=0, but the B0=1 chain can run the addition operation to generate signal output; when only ss6(1,0) is input, ss6 is combined with B0=1, However, the B0=0 chain can perform addition operations and generate signal output; when ss5 and ss6 are input at the same time (1,1), ss5 and ss6 are combined with the two B0 chains respectively, and addition operations cannot occur, and there is no signal output; when the input signal It is (0,0), that is, when ss5 and ss6 are not added, the addition operation in the substrate can run, and the signal output can be generated.

[0214] 1) Truth table:

[0215]

[0216]

[0217] 2) Input strand ssDNA design: According to the desig...

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Abstract

The invention relates to a DNA molecular logic gate based on nucleic acid hybridization. The invention specifically provides a DNA molecular logic gate based on nucleic acid hybridization, the DNA molecular logic gate comprises an input signal, a signal conversion unit and an output signal, the input signal comprises at least two DNA input chains; the signal conversion unit comprises: a substrate chain; the output signal comprises a nucleic acid amplification result. Based on DNA self-assembly, a simple and feasible scheme is provided for construction of a basic principle of a DNA computer.

Description

technical field [0001] The invention belongs to the technical field of molecular computing, and in particular relates to a DNA molecular logic gate based on nucleic acid hybridization. Background technique [0002] In silico creation of molecular biology has attracted a lot of attention, especially in the field of synthetic biology. [0003] Computing equipment mainly includes algorithmic logic unit, control unit, memory, input and output devices. Boolean logic and logic gates are the core of its operation. If biological computers are to become a reality, then the construction of biomolecular logic gates is a must. [0004] At present, with the development of complex biological tools, logic systems based on nucleic acids and proteins have also been produced, and catalysts and logic gates based on DNA and RNA have also been proposed as general components of synthetic chemical circuits and applied to Embedded control of medical treatment, nanotechnology and chemical reaction...

Claims

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Application Information

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IPC IPC(8): G16B30/20G16B25/20G16B15/00
CPCG16B30/20G16B15/00G16B25/20
Inventor 王志敏师咏勇潘敦孙伯宁温燕勤虞修简
Owner SHANGHAI IND TECH INST
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