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Application of small molecule compound piperifoside in preparation of SARS-CoV-2 resisting medicine

A small molecule compound, a new technology of perifox, applied in the field of medicine, can solve the problems of limited clinical efficacy and achieve the effect of low cytotoxicity

Pending Publication Date: 2022-05-20
THE NAVAL MEDICAL UNIV OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although remdesivir has received emergency use authorization from the U.S. Food and Drug Administration for the treatment of COVID-19, its clinical efficacy is relatively limited

Method used

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  • Application of small molecule compound piperifoside in preparation of SARS-CoV-2 resisting medicine
  • Application of small molecule compound piperifoside in preparation of SARS-CoV-2 resisting medicine
  • Application of small molecule compound piperifoside in preparation of SARS-CoV-2 resisting medicine

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Experimental program
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Effect test

Embodiment 1

[0021] 1. Experimental drugs, reagents and materials

[0022] 1. Compounds: Small molecule drug library (product number: L1300-Z349373) approved by the US FDA for clinical use, containing 2580 small molecule compounds, purchased from Selleck, USA, all dissolved in DMSO at a concentration of 10 μM. Remdesivir, purchased from Selleck, USA, was dissolved in DMSO at a concentration of 10 μM.

[0023] 2. African green monkey kidney cells VeroE6 and human lung adenocarcinoma cells Calu-3 were purchased from the Shanghai Cell Institute of the Chinese Academy of Sciences and preserved by the Department of Biomedical Protection, Department of Naval Medicine, Naval Medical University of the Chinese People's Liberation Army.

[0024] 3. DMEM complete cell culture medium, containing 10% fetal calf serum, 0.03% glutamine, non-essential amino acids, ampicillin and streptomycin 100U / mL, adjust the pH to 7.4.

[0025] 4. Cell digestion solution, containing 0.25% trypsin, prepared with phosph...

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Abstract

The invention discloses an application of a small molecule compound piperifoside in preparation of a medicine for resisting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the medicine for resisting SARS-CoV-2 is a medicine composition taking piperifoside as a unique active ingredient or containing piperifoside, and the medicine for resisting SARS-CoV-2 refers to a medicine for preventing or treating SARS-CoV-2 infection. According to the invention, a susceptible cell line of SARS-CoV-2, including African green monkey kidney cells Vero E6 and human lung adenocarcinoma cells Clu-3, is utilized to detect the anti-SARS-CoV-2 activity of piperifen. Experimental results show that the piperifoside can effectively inhibit infection of the SARS-CoV-2 on the susceptible cells, is relatively low in cytotoxicity, is expected to be used as a medicine for effectively resisting SARS-CoV-2 infection, and has an application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of the small molecular compound perifosine in the preparation of anti-SARS-CoV-2 drugs. Background technique [0002] The small molecule compound Perifosine is a new type of Akt inhibitor, which targets the pleckstrin homology region of Akt, and has been approved by the US FDA for clinical use. It has a significant inhibitory effect on immortalized keratinocytes (HaCaT cells) and head and neck squamous carcinoma cells; there is no literature reporting its role in anti-SARS-CoV-2. [0003] The new chemical structure of perifo is as follows (CASNo.157716-52-4): [0004] [0005] Severe acute respiratory syndrome coronavirus 2 (Severe acute respiratory syndrome coronavirus 2, SARS-CoV-2), is a new highly infectious and highly pathogenic infectious disease pathogen, which mainly passes through the respiratory tract through droplets, close contact, etc...

Claims

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Application Information

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IPC IPC(8): A61K31/685A61P11/00A61P31/14
CPCA61K31/685A61P11/00A61P31/14
Inventor 赵平彭浩然丁翠玲任浩赵兰娟郑旭刘燕戚中田
Owner THE NAVAL MEDICAL UNIV OF PLA
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