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Application of mixed bacteria in kelp and gulfweed microbial fermentation feed and preparation method of mixed bacteria

A technology of microbial fermentation and mixed bacteria, applied in animal feed, animal feed, application, etc.

Pending Publication Date: 2022-05-27
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with animal husbandry, the application of bioactive feed in aquaculture is less, in addition, there are few reports in sea cucumber culture

Method used

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  • Application of mixed bacteria in kelp and gulfweed microbial fermentation feed and preparation method of mixed bacteria
  • Application of mixed bacteria in kelp and gulfweed microbial fermentation feed and preparation method of mixed bacteria
  • Application of mixed bacteria in kelp and gulfweed microbial fermentation feed and preparation method of mixed bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of Fermented Seed Liquid

[0051] (1) Activation of strains: Take a small amount of strains from Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus similis, Lactobacillus plantarum and Saccharomyces cerevisiae strain slanted medium with an inoculating needle and apply them to each strain. cultured in the liquid seed medium (primary strain) for 48h at a constant temperature. Bacillus was placed at 36°C, 180r / min shaker for shaking culture; Lactobacillus plantarum was placed at 36°C for static culture; Saccharomyces cerevisiae was placed at 28°C, 180r / min shaker for culture. Then, inoculate the above-mentioned cultivated liquid seed medium (primary strain) into the liquid seed medium of various strains at an inoculum of 1% for 24-48h to expand the culture to make a fermented seed liquid (secondary strain) , the bacterial concentration is not less than 10 9 cfu / L.

[0052] (2) Preparation of spore bacteria suspension: i...

Embodiment 2

[0055] Example 2 Solid state fermentation method

[0056] The solid-state fermentation method is as follows: fully mix the components evenly, add deionized water according to the ratio of material to water, so that the fermented feed can be held in a lump, and the ground is loose, the pH is natural, and sterilized at 121 °C for 20 minutes to obtain solid-state fermentation raw materials; The solid fermentation raw materials are divided into conical flasks, deionized water is added according to an appropriate ratio of material to water, and sterilized at 121 ° C for 20 minutes; after cooling, the prepared bacterial strain fermentation seed liquid is inoculated into the fermentation raw material according to the inoculation ratio. In the base, 30 ℃ constant temperature static fermentation culture for a quantitative time, after fermentation and culture, stir and breathe on the ultra-clean workbench; after the fermentation, dry it in a 50 ℃ constant temperature box, and use a pulve...

Embodiment 3

[0057] Example 3 Screening of excellent strains for solid-state fermentation

[0058] 7 kinds of probiotics: Lactobacillus plantarum, Bacillus similis, Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Aspergillus niger as fermentation strains. The basic fermentation parameters of kelp feed were set as follows: temperature 30°C, time 72h, inoculum volume 10%, and material-water ratio 1:1.2. The basic fermentation parameters of sargassum feed were as follows: temperature 30°C, time 72h, inoculum amount 10%, and material-water ratio 1:1. After the fermentation was completed, the excellent single bacteria were screened out with crude protein, soluble protein and algin content as indicators.

[0059] Depend on Figure 1-2 It can be seen that after fermentation with different bacteria, the crude protein content of the kelp feed treatment group did not change significantly, among which the fermentation effect of Bacillus subtilis w...

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Abstract

The invention discloses application of mixed bacteria in a kelp and gulfweed microbial fermented feed and a preparation method of the mixed bacteria, relates to the technical field of holothurian culture, and in particular relates to a biological holothurian compound feed. According to the invention, lactobacillus plantarum, bacillus siamensis, bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, saccharomyces cerevisiae and aspergillus niger are used as fermentation strains, strains most suitable for fermentation of kelp and gulfweed are obtained through screening, a mixed fermentation optimization experiment is carried out, a fermentation process is established, and the fermentation efficiency is improved. And an experimental foundation is laid for application of seaweed alternative resources in holothurian culture.

Description

technical field [0001] The invention relates to the technical field of sea cucumber culture, in particular to a biological sea cucumber compound feed. Background technique [0002] With the rapid development of sea cucumber aquaculture, the problem of single and lack of sea cucumber bait is gradually exposed. The large seaweeds Sargassum and Sageweed are commonly used natural bait for sea cucumber farming. With the rapid development of my country's coastal sea cucumber farming industry, natural sea cucumber bait is due to its low output, high cost and limited by its farming environment and conditions and other factors. It has been unable to meet the needs of the rapid development of sea cucumber aquaculture. Therefore, it is urgent to find alternative resources of seaweed and develop new high-nutrition and low-cost sea cucumber compound feed. [0003] Microbial fermented feed is a kind of biological feed with rich nutrition, good palatability and high content of viable bact...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/30A23K10/12A23K10/37A23K10/26
CPCA23K50/80A23K10/30A23K10/12A23K10/37A23K10/26A23V2400/169Y02P60/87
Inventor 于基成赵美娟刘秋戴祥英徐俊科刘新宇
Owner DALIAN NATIONALITIES UNIVERSITY
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